Nano-sized Indocyanine Green J-aggregate as a One-component Theranostic Agent.
Nano-sized Indocyanine Green J-aggregate as a One-component Theranostic Agent.
复制标题
作者：
Liu R;Tang J;Xu Y;Zhou Y;Dai Z
近年来，开发生物相容的高效光热耦合剂（PCA）用于肿瘤的图像引导光热治疗受到了越来越多的关注。虽然已经开发了各种PCA，但这些材料的临床转化在很大程度上受到潜在生物安全问题和扩大生产的挑战的阻碍。在这项研究中，我们提出了纳米尺寸的吲哚菁绿色（ICG）J-聚集体（IJA）作为一个有前途的PCA，这是一个非常简单的方法，使用临床批准的ICG作为唯一的赋形剂。所制备的IJA在各种溶液中保持稳定，并且与游离ICG相比，其吸收峰红移约115 nm。重要的是，IJA可以在内化到细胞中后再次解离成游离ICG，并且表现出与ICG相当的高生物安全性。IJA在体外和体内均表现出良好的光热治疗效果。此外，IJA还可以作为良好的光声造影剂和内化响应荧光探针。IJA具有制备简便、安全性高、治疗诊断效果好等优点，有望成为一种单组分肿瘤治疗诊断剂。
The development of biocompatiable efficient photothermal coupling agent (PCA) for image-guided photothermal therapy of cancer has gained increasing interests in recent years. Although various PCAs have been developed, the clinical translations of these materials have been largely hindered by the potential biosafety issues and challenges of scaling-up manufactures. In this research, we proposed nano-sized indocyanine green (ICG) J-aggregate (IJA) as a promising PCA which is fabricated by a very facile method using clinical-approved ICG as the only excipient. The as-prepared IJA remains stable in various solution and shows a ~115 nm red-shift in absorption peak compared to free ICG. Importantly, IJA can be disassociated into free ICG again after internalized into cells and exhibits high biosafety comparable to ICG. The IJA performs well for photothermal therapy both in vitro and in vivo. In addition, the IJA can also be used as a good photoacoustic contrast agent and internalization-responsive fluorescence probe. The facile preparation, high safety and excellent theranostic performance indicated that IJA might be a promising one-component agent for cancer theranostics.