Identification of bacterial microflora in the midgut of the larvae and adult of wild caught Anopheles stephensi: A step toward finding suitable paratransgenesis candidates
Identification of bacterial microflora in the midgut of the larvae and adult of wild caught Anopheles stephensi: A step toward finding suitable paratransgenesis candidates
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DOI：
10.1016/j.actatropica.2011.10.015
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发表时间：
2012-02-01
期刊：
影响因子：
2.7
通讯作者：
Ghoorchian, Sadigheh
中科院分区：
文献类型：
--
作者：
Chavshin, Ali Reza;Oshaghi, Mohammad Ali;Ghoorchian, Sadigheh
为了描述中肠微生物的多样性，寻找对疟疾传播不敏感的副转基因按蚊的遗传操作候选细菌，采用常规无细胞培养技术和16S核糖体RNA(RRNA)基因文库分析，研究了伊朗南部野生斯氏按蚊幼虫和成虫中肠微生物区系。幼虫中肠微生物区系共鉴定出革兰氏阴性杆菌、金黄色杆菌、气单胞菌、假单胞菌、克雷伯氏菌、肠杆菌和希瓦氏杆菌等12属40种，革兰氏阳性杆菌、肠球菌、革兰氏杆菌、微杆菌和红球菌5种。对成年中肠微生物区系的分析表明，存在25种革兰氏阴性菌，分别属于假单胞菌、产碱杆菌、波氏杆菌、甲藻和气单胞菌。中肠共生菌以假单胞菌和外向杆菌为主，幼虫期分别为51%和14%，成虫期分别为54%和20%。假单胞菌。从幼虫期和成虫期均分离到气单胞菌和甲虫属，提示可能从幼虫期传播到成虫期。在廉价的培养基中快速生长，革兰氏阴性，在幼虫和成虫阶段都有优势，以及在其他疟疾媒介中的存在，使假单胞菌成为一种合适的副转基因蚊子。斯氏杆菌和其他疟疾媒介。(C)2011爱思唯尔B.V.保留所有权利。
To describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic Anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult Anopheles stephensi mosquito midgut from southern Iran was studied using a conventional cell-free culture technique and analysis of a 16S ribosomal RNA (rRNA) gene sequence library. Forty species in 12 genera including seven Gram-negative Myroides, Chryseobacterium, Aeromonas, Pseudomonas, Klebsiella, Enterobacter and Shewanella and five Gram-positive Exiguobacterium, Enterococcus, Kocuria, Microbacterium and Rhodococcus bacteria were identified in the microbiota of the larvae midgut. Analysis of the adult midgut microbiota revealed presence of 25 Gram-negative species in five genera including Pseudomonas, Alcaligenes, Bordetella, Myroides and Aeromonas. Pseudomonas and Exiguobacterium with a frequency of 51% and 14% at the larval stage and Pseudomonas and Aeromonas with a frequency of 54% and 20% at the adult stage were the most common midgut symbionts. Pseudomonas. Aeromonas and Myroides genera have been isolated from both larvae and adult stages indicating possible trans-stadial transmission from larva to adult stage. Fast growth in cheap media, Gram negative, and being dominantly found in both larvae and adult stages, and presence in other malaria vectors makes Pseudomonas as a proper candidate for paratransgenesis of An. stephensi and other malaria vectors. (C) 2011 Elsevier B.V. All rights reserved.