CHEMILUMINESCENT ENZYME METHOD FOR GLUCOSE
CHEMILUMINESCENT ENZYME METHOD FOR GLUCOSE
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DOI：
10.1021/ac60352a008
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发表时间：
1975-01-01
影响因子：
7.4
通讯作者：
MALOY, JT
中科院分区：
文献类型：
--
作者：
AUSES, JP;COOK, SL;MALOY, JT
在此测定中，在pH 7的葡萄糖氧化酶存在下，葡萄糖的氧化提供了H2 O2的来源，而H2 O2又在3-氨基邻苯二甲酰肼存在下与Fe（CN）63~反应，产生与初始葡萄糖浓度成正比的荧光（CL）。只有在碱性溶液中才能观察到CL发射，用高容量的氨缓冲液迅速调节溶液的pH值，从高酶活性水平到可检测的CL发射水平。酶活性5分钟后获得的积分CL信号与10 µ样品中的葡萄糖浓度在普通临床感兴趣的整个范围（2-200 mg/dl）内呈线性关系。由于该方法依赖于发射过程而不是吸收过程，因此可用于生物组织中少量葡萄糖的快速测定。
In this determination, the oxidation of glucose in the pres-ence of glucose oxidase at pH 7 provides a source for H202 which, in turn, reacts with Fe (CN) 63~ in the presence of lu-minol (3-aminophthalhydrazide) to produce chemilumines-cence (CL) proportional to the initial glucose concentration. CL emission is observed only in basic solution, and an am-monia buffer of high capacity Is used to rapidly adjust the pH of the solution from levels of high enzyme activity to lev-els of detectable CL emission. The integrated CL signal ob-tained after five minutes of enzyme activity was linear with glucose concentration in 10-µ samples over the entire range of ordinary clinical interest (2-200 mg/dl). Because the method relies on an emissive process rather than ab-sorbance, it may find applicability in the rapid determination of small quantities of glucose in biological tissues.