CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm
CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm
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DOI：
10.1073/pnas.2536658100
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发表时间：
2003-12-09
影响因子：
11.1
通讯作者：
Babcock, DF
中科院分区：
文献类型：
--
作者：
Carlson, AE;Westenbroek, RE;Babcock, DF
CatSper家族蛋白是推定的离子通道，仅在精子鞭毛的膜中表达，并且是男性生育力所需的。在这里，我们表明，小鼠CatSper 1是必不可少的去极化诱发的Ca 2+的入口和超活化运动，一个关键的鞭毛功能。CatSper 1不需要其他发育标志，包括Ca（V）1.2，Ca（V）2.2和Ca（V）2.3离子通道蛋白的区域分布，cAMP介导的HCO 3-激活运动，以及精子获能的蛋白磷酸化级联反应。我们建议，CatSper 1的功能作为一个电压门控的Ca 2+通道，控制Ca 2+的进入，以介导的超激活运动所需的后期准备精子受精。
CatSper family proteins are putative ion channels expressed exclusively in membranes of the sperm flagellum and required for male fertility. Here, we show that mouse CatSper1 is essential for depolarization-evoked Ca2+ entry and for hyperactivated movement, a key flagellar function. CatSper1 is not needed for other developmental landmarks, including regional distributions of Ca(V)1.2, Ca(V)2.2, and Ca(V)2.3 ion channel proteins, the cAMP-mediated activation of motility by HCO3-, and the protein phosphorylation cascade of sperm capacitation. We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization.