Reconstruction of the P2X2 receptor reveals a vase-shaped structure with lateral tunnels above the membrane
Reconstruction of the P2X2 receptor reveals a vase-shaped structure with lateral tunnels above the membrane
复制标题
P2X2 受体的重建揭示了膜上方有横向隧道的花瓶状结构
DOI：
10.1016/j.str.2008.12.007
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发表时间：
2009
期刊：
影响因子：
5.7
通讯作者：
Yoshihiro Kubo and Chikara Sato
中科院分区：
文献类型：
--
作者：
Kazuhiro Mio;Toshihiko Ogura;Tomomi Yamamoto;Yoko Hiroaki;Yoshinori Fujiyoshi;Yoshihiro Kubo and Chikara Sato
响应于细胞间信使ATP，P2X受体传递各种感觉信息，包括疼痛。在这里，我们已经重建了P2X2受体的结构，分辨率为15 μ m，来自90，000多个粒子图像，这些图像是用配备氦冷却台的冷冻电子显微镜拍摄的。该三维描绘（假定处于闭合状态）揭示了高度为200厘米且大直径为160厘米的细长花瓶形结构202。细胞外和跨膜结构域呈现两层结构，其中稀疏的外层包围成孔的内部密度。在膜中间的假定离子传导通路的直径减小被认为是孔的最西部，这已经从电生理学研究中预测。P2X2受体的稀疏延伸结构表明亚基的松散组装，这可能是P2X受体活化依赖性孔扩张的基础。
In response to the intercellular messenger ATP, P2X receptors transfer various sensory information, including pain. Here we have reconstructed the structure of the P2X2receptor at 15 Å resolution from more than 90,000 particle images, taken with a cryo-electron microscope equipped with a helium-cooled stage. This three-dimensional depiction, presumably in a closed state, revealed an elongated vase-shaped structure 202 Å in height and 160 Å in major diameter. The extracellular and transmembrane domains present a two-layered structure, in which a sparse outer layer surrounds a pore-forming inner density. The decreased diameter of a putative ion-conducting pathway at the middle of the membrane was considered to be the narrowest part of the pore, which has been predicted from electrophysiological studies. The sparse, extended structure of the P2X2receptor indicates a loose assembly of subunits, which could be a basis for the activation-dependent pore dilation of P2X receptors.