New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB
New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB
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DOI：
10.1101/gad.12.1.34
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发表时间：
1998-01-01
影响因子：
10.5
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Lagrange, T;Kapanidis, AN;Ebright, RH
直接位于TATA元件上游并具有共有序列5 ′-G/C-G/C-G/A-C-G-C-C-3 ′的序列元件影响转录因子IIB进入转录复合体并支持转录起始的能力。该序列元件直接被转录因子IIB识别，识别涉及IIB的α-螺旋4'和5'，其包含螺旋-转角-螺旋DNA结合基序。这些观察结果表明，转录起始除了TATA元件、起始元件和下游启动子元件之外，还涉及第四个tore启动子元件，IIB识别元件（BRE），并且除了转录因子IID之外，还涉及第二个序列特异性通用转录因子IIB。
A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB, Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth tore promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.