SERIAL ANALYSIS OF MICROTUBULES IN CULTURED RAT SENSORY AXONS
SERIAL ANALYSIS OF MICROTUBULES IN CULTURED RAT SENSORY AXONS
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DOI：
10.1007/bf01262592
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
BUNGE, MB
中科院分区：
文献类型：
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作者：
BRAY, D;BUNGE, MB
在大鼠背根神经节的器官型培养物中的轴突束在190个连续的横截面中进行分析，总束长度计算为12.8 μ m。观察微管的数量、位置、连续性和滑面内质网（SER）的组成。在27个轴突中追踪的187个微管显示了32个终止系列;其中14个是开始，18个是停止，与远离神经元索马的进展有关，3个微管开始和结束系列。微管总体上是长的，它们的长度计算为平均108 μ m。终止通常发生在质膜附近，但在其他方面没有表现出明显的形态特化。在一些轴突中的微管集中在轴突的外围，一个区域，从其中神经丝被排除在外。在整个系列中，轴突中小管的相对位置没有以任何系统的方式（例如，通过旋转）改变。SER在形式上表现出明显的变化，从预期的膜结合囊泡到类似于微管或更细的染色细丝的轮廓。
A fascicle of axons in an organotypic culture of rat dorsal root ganglion was analyzed in 190 serial transverse sections, a total fascicle length calculated to be 12.8 .mu.m. The number, position and continuity of microtubules and components of smooth endoplasmic reticulum (SER) were examined. The 187 microtubules traced in 27 axons showed 32 terminations in the series; 14 of these were starts and 18 were stops in relation to progression away from the neuronal soma, 3 tubules began and ended in the series. Microtubules overall were long, their lengths calculated to average 108 .mu.m. Terminations usually occurred close to the plasma membrane but otherwise exhibited no obvious morphological specialization. Microtulbles in some axons were concentrated in the periphery of the axon, a region from which neurofilaments were excluded. The relative position of tubules in the axon did not change in any systematic way (for example, by rotation) throughout the series. The SER showed marked variation in form, from the expected membrane-bounded vesicle to profiles that resembled a microtubule or a thinner densely stained filament.