Metabolic voxel-based analysis of the complete human brain using fast 3D-MRSI: Proof of concept in multiple sclerosis.
Metabolic voxel-based analysis of the complete human brain using fast 3D-MRSI: Proof of concept in multiple sclerosis.
复制标题
DOI：
10.1002/jmri.25139
复制
发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Ranjeva JP
中科院分区：
文献类型：
--
作者：
Donadieu M;Le Fur Y;Lecocq A;Maudsley AA;Gherib S;Soulier E;Confort-Gouny S;Pariollaud F;Ranjeva MP;Pelletier J;Guye M;Zaaraoui W;Audoin B;Ranjeva JP
利用优化的快速体积回波平面光谱成像（3D-EPSI），我们旨在检测多发性硬化症患者整个人脑的局部代谢异常。对3T时AC-PC和AC-PC+15°轴向面覆盖全脑的2个3D-EPSI进行加权平均组合，获得5种代谢物的高质量代谢物图谱：n -乙酰天冬氨酸（NAA）、谷氨酸+谷氨酰胺（Glx）、胆碱（Cho）、肌醇（m-Ins）和肌酸+磷酸肌酸（tCr）。空间归一化后，19名复发缓解型多发性硬化症患者的图谱与19名匹配的对照组进行比较，使用统计作图分析来确定代谢异常的地形。生成概率白质（WM） T2病变图和灰质（GM）萎缩图。两组方差分析（SPM8, p<0.005， FDR校正p<0.05，以年龄和性别为混杂协变量）比较年龄和性别匹配的患者和对照组，结果显示代谢物水平异常的集群：1)NAA（约- 15%）和Glx（约20%）减少，主要发生在前额皮质、运动皮质、双侧丘脑和内侧颞叶皮质的GM中，与神经元/神经星形细胞功能障碍一致；ii) wmt2病变内和颞枕叶正常WM内m-Ins增加（约+20%），提示神经胶质激活。我们展示了在整个大脑（从顶点到小脑）上进行无创绘制的能力，并验证了与MS相关的代谢异常序列，以表征影响WM和GM广泛区域的病理过程的地形及其功能影响。
Using optimized fast volumic echo planar spectroscopic imaging (3D-EPSI), we aimed to detect local metabolic abnormalities over the complete human brain in multiple sclerosis patients. Weighted mean combination of two 3D-EPSI covering the whole brain acquired at 3T in AC-PC and AC-PC+15° axial planes was performed to obtain high quality metabolite maps for five metabolites: N-acetyl aspartate (NAA), glutamate+glutamine (Glx), choline (Cho), myo-inositol (m-Ins) and creatine+phosphocreatine (tCr). After spatial normalisation, maps from 19 patients suffering from relapsing-remitting multiple sclerosis were compared to 19 matched controls using statistical mapping analyses to determine the topography of metabolic abnormalities. Probabilistic white matter (WM) T2 lesion maps and grey matter (GM) atrophy maps were also generated. Two-group ANOVA (SPM8, p<0.005, FDR corrected p<0.05 at the cluster level with age and sex as confounding covariates) comparing Patients and controls matched for age and sex showed clusters of abnormal metabolite levels with i) decreased NAA (around −15%) and Glx (around 20%) predominantly in GM within prefrontal cortices, motor cortices, bilateral thalami and mesial temporal cortices in line with neuronal/neuro-astrocytic dysfunction, ii) increased m-Ins (around +20%) inside WM T2 lesions and in the normal appearing WM of temporal-occipital lobes suggesting glial activation. We demonstrated the ability to map non-invasively over the complete brain - from vertex to cerebellum – with a validated sequence, the metabolic abnormalities associated with MS, for characterizing the topography of pathological processes affecting widespread areas of WM and GM and its functional impact.