Phenotypic diversity of neoplastic chondrocytes and extracellular matrix gene expression in cartilaginous neoplasms.
Phenotypic diversity of neoplastic chondrocytes and extracellular matrix gene expression in cartilaginous neoplasms.
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软骨肿瘤中肿瘤性软骨细胞的表型多样性和细胞外基质基因表达。
DOI：
--
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发表时间：
1997
影响因子：
6
通讯作者：
Thomas Kirchner
中科院分区：
文献类型：
--
作者：
Thomas Aigner;Susanne Dertinger;Silvia Isolde;Vornehm;Jayesh;Dudhia;K. V. D. Mark;Thomas Kirchner
软骨细胞分化的特征在于不同的细胞表型，其可以通过特异性细胞外基质基因表达谱来鉴定。通过对一系列良性和恶性人类软骨源性肿瘤的mRNA和蛋白质水平进行原位分析，我们首次能够在体内鉴定肿瘤软骨细胞的不同表型：1）成熟软骨细胞，其合成特征性软骨细胞外肿瘤基质，2）类似于胎儿生长板的肥大软骨细胞的细胞，3）类似于所谓的去分化软骨细胞的细胞，和4）分化良好的软骨细胞，其表达I型胶原，表明存在肥大后分化的肿瘤软骨细胞。软骨细胞表现出一系列的表型被发现存在于同一肿瘤。观察到的不同表型，包括去分化表型，与高级软骨肉瘤的间变性细胞相反。表达数据与肿瘤形态的比较揭示了细胞表型、肿瘤基质组成、肿瘤内基质和细胞形态之间的关系。肿瘤性软骨细胞的明显不同的表型是软骨样肿瘤的特征性高生化和形态异质性的基础，并揭示了它们的生物学和临床行为。
Chondrocyte differentiation is characterized by distinct cellular phenotypes, which can be identified by specific extracellular matrix gene expression profiles. By applying in situ analysis on the mRNA and protein level in a series of benign and malignant human chondrogenic neoplasms, we were able to identify for the first time different phenotypes of neoplastic chondrocytes in vivo: 1) mature chondrocytes, which synthesized the characteristic cartilaginous extracellular tumor matrix, 2) cells resembling hypertrophic chondrocytes of the fetal growth plate, 3) cells resembling so-called dedifferentiated chondrocytes, and 4) well differentiated chondrocytic cells, which expressed type I collagen, indicating the presence of post-hypertrophic differentiated neoplastic chondrocytes. Chondrocytes exhibiting a range of phenotypes were found to be present in the same neoplasm. The different observed phenotypes, including the dedifferentiated phenotype, were in contrast to the anaplastic cells of high-grade chondrosarcomas. Comparison of expression data with tumor morphology revealed a relationship between the cellular phenotypes, the tumor matrix composition, and the matrix and cell morphology within the neoplasms. The distinctly different phenotypes of neoplastic chondrocytes are the basis of the characteristic high biochemical and morphological heterogeneity of chondroid neoplasms and shed light on their biological and clinical behavior.
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病毒转化对培养的胚胎鸡软骨细胞合成和分泌胶原蛋白和纤连蛋白样分子的影响。
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yoshimura,M;Jimenez,SA;Kaji,A
通讯作者：
Kaji,A
鸡软骨和玻璃体液中 IX 型胶原蛋白多糖的结构和功能比较。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Brewton,RG;Wright,DW;Mayne,R
通讯作者：
Mayne,R
影响因子：
15.9
作者：
GOLDRING, MB;BIRKHEAD, JR;APPERLEY, JF
通讯作者：
APPERLEY, JF
DOI：
10.1016/s0021-9258(18)98361-4
发表时间：
1993-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Richard Mayne;R. G. Brewton;P. M. Mayne;John Baker
通讯作者：
Richard Mayne;R. G. Brewton;P. M. Mayne;John Baker