A Novel Odor Stimulation System in Freely Moving, Behaving Animals
A Novel Odor Stimulation System in Freely Moving, Behaving Animals
复制标题
一种针对自由活动、行为动物的新型气味刺激系统
DOI：
10.1111/1749-4877.12674
复制
发表时间：
--
影响因子：
3.3
通讯作者：
An-An Li
中科院分区：
文献类型：
--
作者：
Xin-Song Guo;Shan Li;Xue-Jie Yu;Ting-Ting Wu;Peng-Lai Liu;Yu-Feng Shao;An-An Li
准确可靠地向动物呈现气味对于嗅觉研究至关重要。虽然气味刺激系统在麻醉或清醒，头部固定的动物是很好的建立，时间精确的气味呈现清醒，自由移动的动物仍然是一个挑战。在这里，我们描述了一种新的气味刺激系统，直接向自由移动的小鼠的鼻孔提供气味。该系统包括三个模块：气味输送模块、气味产生模块和控制模块。新系统精确且时间可靠，气味刺激可以由特定的嗅探阶段或其他事件触发。此外，该系统可以与神经记录（如电生理学）和嗅觉行为测试相结合，以研究大脑中的神经元如何在个体嗅觉行为期间代表气味信息。这种创新的气味刺激系统可能会取代传统的刺激系统：它将使精确的气味呈现在广泛的嗅觉研究在自由移动的动物。本文受版权保护。All rights reserved.
Precise and reliable presentation of odorants to animals is crucial for olfactory studies. Although odor stimulation systems in anesthetized or awake, head-fixed animals are well established, temporally precise odor presentation in awake, freely moving animals remains a challenge. Here, we describe a new odor stimulation system which presents odors directly to the nostrils of freely moving mice. The system comprises three modules: an odor-delivery module, an odor-generation module, and a control module. The new system is precise and temporally reliable, and odor stimulation can be triggered by specific sniffing phases or other events. Moreover, the system can be combined with neural recordings, such as electrophysiology, and olfactory behavioral tests to investigate how neurons in the brain represent odor information during individual olfactory behaviors. This innovative odor stimulation system may replace traditional stimulation systems: it will enable precise odor presentation in a wide range of olfactory studies in freely moving animals. This article is protected by copyright. All rights reserved.