Regulation of neuronal oxytocin mRNA by ovarian steroids in the mature and developing hypothalamus.
Regulation of neuronal oxytocin mRNA by ovarian steroids in the mature and developing hypothalamus.
复制标题
成熟和发育的下丘脑中卵巢类固醇对神经元催产素 mRNA 的调节。
DOI：
10.1073/pnas.86.7.2468
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发表时间：
1989
影响因子：
11.1
通讯作者：
Bloom,FE
中科院分区：
文献类型：
--
作者：
Miller,FD;Ozimek,G;Milner,RJ;Bloom,FE
我们研究了围产期和成熟雌性大鼠催产素mRNA神经元表达的变化，这是内源性性腺类固醇的作用。Northern印迹分析表明，随着青春期的发育，女性大脑中催产素mRNA的丰度显著增加。成年女性摘除卵巢后，脑内催产素的总mRNA水平显著降低。相比之下，哺乳期母亲的神经元催产素mRNA水平增加。对青春期、成熟期处女和去卵巢处女脑神经元催产素mRNA的原位杂交分析表明，表达催产素mRNA的神经元的位置和数量没有变化，总的脑催产素mRNA的差异归因于每个神经元表达的量。在整个下丘脑的催产素神经元中发现了不同的mRNA丰度，包括那些被认为投射到神经垂体的大细胞神经元，以及那些被认为完全建立脑内联系的微小细胞起源的神经元。性腺成熟过程中催产素mRNA水平的这种发育和动态调节可能与该肽对雌性大鼠生殖生物学的外周和中枢作用相协调。
We have examined the changes in neuronal expression of oxytocin mRNA in the perinatal and mature female rat as a function of endogenous gonadal steroids. Northern blot analysis demonstrated a significant developmental increase in the abundance of oxytocin mRNA in the female brain concomitant with puberty. Ovariectomy of adult females decreased total brain oxytocin mRNA to significantly lower levels. In contrast, lactating mothers had increased levels of neuronal oxytocin mRNA. In situ hybridization analysis of neuronal oxytocin mRNA in adolescent, mature virgin, and ovariectomized virgin female brains demonstrated that the location and number of neurons expressing oxytocin mRNA was unchanged and that total brain oxytocin mRNA differences were attributable to amounts expressed per neuron. Differences in mRNA abundance were noted in oxytocin neurons throughout the hypothalamus, including those known to project as magnocellular neurons to the neurohypophysis and those of parvocellular origin thought to make wholly intracerebral connections. This developmental and dynamic regulation of oxytocin mRNA levels during gonadal maturation may coordinate the peripheral and central effects of this peptide on the reproductive biology of the female rat.