Overexpression of GRP78 and GRP94 are markers for aggressive behavior and poor prognosis in gastric carcinomas
Overexpression of GRP78 and GRP94 are markers for aggressive behavior and poor prognosis in gastric carcinomas
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DOI：
10.1016/j.humpath.2007.11.009
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发表时间：
2008-07-01
期刊：
影响因子：
3.3
通讯作者：
Takano, Yasuo
中科院分区：
文献类型：
--
作者：
Zheng, Hua-chuan;Takahashi, Hiroyuki;Takano, Yasuo
葡萄糖相关蛋白（GRPs）在内质网中普遍表达，并能协助蛋白质折叠和组装；因此，它们被认为是分子伴侣。GRP78和GRP94在葡萄糖饥饿诱导下表达上调。为了明确这两种分子在胃癌发生和发展中的作用，我们使用GRP78和GRP94抗体对含有胃癌、腺瘤和非肿瘤性粘膜的组织芯片进行免疫组化，并比较其表达与肿瘤的临床病理参数。采用免疫组织化学和Western blot方法对胃癌细胞株（MKN28、AGS、MKN45、KATO-III和HGC-27）进行了两种蛋白的检测。两种蛋白在胃癌和腺瘤组织中的表达均高于非肿瘤组织(P
Glucose-related proteins (GRPs) are ubiquitously expressed in endoplasmic reticulum and able to assist in protein folding and assembly; consequently, they are considered as molecular chaperones. GRP78 and GRP94 expression was induced by glucose starvation and up-regulated in the malignancies. To clarify the roles of both molecules in tumorigenesis and progression of gastric carcinomas, immunohistochemistry was used on tissue microarray containing gastric carcinomas, adenomas, and nonneoplastic mucosa using the antibodies against GRP78 and GRP94, with a comparison of their expression with clinicopathological parameters of carcinomas. Gastric carcinoma cell lines (MKN28, AGS, MKN45, KATO-III, and HGC-27) were studied for both proteins by immunohistochemistry and Western blot. There was more expression of both proteins in gastric carcinoma and adenoma than in nonneoplastic mucosas (P