Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules
Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules
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DOI：
10.1038/90228
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发表时间：
2001-07-01
影响因子：
46.9
通讯作者：
Nie, S
中科院分区：
文献类型：
--
作者：
Han, MY;Gao, XH;Nie, S
通过将不同尺寸的量子点（硫化锌封建的硒化核纳米晶体）嵌入到精确控制比率的聚合物微粒中，可以实现用于生物测定的多色光学编码。它们的新型光学特性（例如，尺寸可调的发射和同时激发）使这些高度发光的量子点（QDS）理想的荧光团用于波长和强度多路复用。使用10个强度水平和6种颜色可以从理论上编码100万个核酸或蛋白质序列。成像和光谱测量结果表明，QD标记的珠子高度均匀且可重现，在有利条件下产生高达99.99％的珠子鉴定精度。 DNA杂交研究表明，可以同时读取编码和目标信号在单个珠子水平上。这种光谱编码技术有望在基因表达研究，高通量筛查和医学诊断方面开放新的机会。
Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots (zinc sulfide-capped cadmium selenide nanocrystals) into polymeric microbeads at precisely controlled ratios. Their novel optical properties (e.g., size-tunable emission and simultaneous excitation) render these highly luminescent quantum dots (QDs) ideal fluorophores for wavelength-and-intensity multiplexing. The use of 10 intensity levels and 6 colors could theoretically code one million nucleic acid or protein sequences. Imaging and spectroscopic measurements indicate that the QD-tagged beads are highly uniform and reproducible, yielding bead identification accuracies as high as 99.99% under favorable conditions. DNA hybridization studies demonstrate that the coding and target signals can be simultaneously read at the single-bead level. This spectral coding technology is expected to open new opportunities in gene expression studies, high-throughput screening, and medical diagnostics.