A FRAMESHIFT MUTATION AFFECTING THE CARBOXYL TERMINUS OF THE SIMIAN VIRUS-40 LARGE TUMOR-ANTIGEN RESULTS IN A REPLICATION-DEFECTIVE AND TRANSFORMATION-DEFECTIVE VIRUS
A FRAMESHIFT MUTATION AFFECTING THE CARBOXYL TERMINUS OF THE SIMIAN VIRUS-40 LARGE TUMOR-ANTIGEN RESULTS IN A REPLICATION-DEFECTIVE AND TRANSFORMATION-DEFECTIVE VIRUS
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DOI：
10.1073/pnas.80.23.7065
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MANLEY, JL
中科院分区：
文献类型：
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作者：
LEWIS, ED;CHEN, S;MANLEY, JL
采用一种新的核苷酸定向诱变方法，在SV40早期区域构建了一个移码突变。突变的DNA指定由. apprx组成的84，000道尔顿大肿瘤抗原。75，000道尔顿由野生型阅读框编码，9000道尔顿由备选阅读框编码（野生型大肿瘤抗原是apprx. 82，000道尔顿）。该蛋白的移码羧基端与多瘤病毒中等大小肿瘤抗原的相同区域具有很强的相似性。当将突变DNA引入允许的猴细胞中时，突变DNA无法复制，并且无法转化非允许的小鼠细胞。
A frameshift mutation in the SV40 early region was constructed using a novel method of oligonucleotide-directed mutagenesis. The mutated DNA specifies an 84,000 dalton large tumor antigen that consists of .apprx. 75,000 daltons encoded by the wild-type reading frame and 9000 daltons by the alternative reading frame (wild-type large tumor antigen is .apprx. 82,000 daltons). The frameshifted carboxyl terminus of the protein bears a strong similarity to the same region of polyoma virus middle-sized tumor antigen. The mutant DNA is unable to replicate when introduced into permissive monkey cells and incapable of transforming nonpermissive mouse cells.