Integration of engineered cartilage
Integration of engineered cartilage
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DOI：
10.1016/s0736-0266(01)00030-4
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发表时间：
2001-11-01
影响因子：
2.8
通讯作者：
Vunjak-Novakovic, G
中科院分区：
文献类型：
--
作者：
Obradovic, B;Martin, I;Vunjak-Novakovic, G
使用牛关节软骨细胞、生物可降解支架和生物反应器在体外工程化的软骨构建体的结构和功能可以通过组织培养的条件和持续时间来调节。我们假设工程软骨的整合特性取决于构建体的发育阶段和邻近软骨的细胞外基质含量，并且可以在受控的体外条件下研究整合的某些方面。盘形结构（培养5 +/- 1天或5 +/- 1周）或外植体（未处理或胰蛋白酶处理的软骨）缝合成环形外植体（未处理的或胰蛋白酶处理的软骨）以形成复合物，在生物反应器中再培养1-8周，并进行生物化学、组织学和机械评价（中心盘的压缩刚度、整合界面的粘合强度）。不成熟的结构有较差的机械性能，但集成更好地比更成熟的结构或软骨外植体。不成熟结构的整合涉及细胞增殖和软骨组织的逐步形成，而更成熟的结构或天然软骨的整合只涉及细胞外基质成分的分泌。整合模式与椎间盘-环界面的粘附强度相关，未成熟结构的粘附强度明显高于更成熟的结构或软骨外植体。胰蛋白酶处理邻近软骨进一步增强未成熟构建体的整合。(C)2001骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs. (C) 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.