Resveratrol and Grape Extract-loaded Solid Lipid Nanoparticles for the Treatment of Alzheimer's Disease.
Resveratrol and Grape Extract-loaded Solid Lipid Nanoparticles for the Treatment of Alzheimer's Disease.
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DOI：
10.3390/molecules22020277
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发表时间：
2017-02-13
期刊：
影响因子：
--
通讯作者：
Pereira MC
中科院分区：
文献类型：
--
作者：
Loureiro JA;Andrade S;Duarte A;Neves AR;Queiroz JF;Nunes C;Sevin E;Fenart L;Gosselet F;Coelho MA;Pereira MC
β 淀粉样肽 (Aβ) 的聚集与神经炎斑块的形成有关，神经炎斑块是阿尔茨海默病 (AD) 的病理标志。多种天然化合物已被建议作为 AD 的治疗剂。在这些化合物中，白藜芦醇因其神经保护特性而引起了人们的极大兴趣。在这里，我们提供了证据表明葡萄皮和葡萄籽提取物增强了对 Aβ 聚集的抑制作用。然而，静脉注射后，白藜芦醇在肝脏和肠上皮细胞中迅速代谢为葡萄糖醛酸和酚基的硫酸盐结合物（不到2小时），然后被消除。在本研究中，我们表明，用抗体（抗转铁蛋白受体单克隆抗体（OX26 mAb））功能化的固体脂质纳米颗粒（SLN）可以作为一种可能的载体，将提取物转运至大脑。对人脑样内皮细胞的实验表明，OX26 SLN 的细胞摄取效率明显高于正常 SLN 和用非特异性抗体功能化的 SLN。因此，当用 OX-26 功能化时，这些不同 SLN 的转胞吞能力更高。
The aggregation of amyloid-β peptide (Aβ) has been linked to the formation of neuritic plaques, which are pathological hallmarks of Alzheimer’s disease (AD). Various natural compounds have been suggested as therapeutics for AD. Among these compounds, resveratrol has aroused great interest due to its neuroprotective characteristics. Here, we provide evidence that grape skin and grape seed extracts increase the inhibition effect on Aβ aggregation. However, after intravenous injection, resveratrol is rapidly metabolized into both glucuronic acid and sulfate conjugations of the phenolic groups in the liver and intestinal epithelial cells (within less than 2 h), which are then eliminated. In the present study, we show that solid lipid nanoparticles (SLNs) functionalized with an antibody, the anti-transferrin receptor monoclonal antibody (OX26 mAb), can work as a possible carrier to transport the extract to target the brain. Experiments on human brain-like endothelial cells show that the cellular uptake of the OX26 SLNs is substantially more efficient than that of normal SLNs and SLNs functionalized with an unspecific antibody. As a consequence, the transcytosis ability of these different SLNs is higher when functionalized with OX-26.
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影响因子：
2.1
作者：
Loureiro, Joana A.;Rocha, Sandra;Pereira, Maria do Carmo
通讯作者：
Pereira, Maria do Carmo
影响因子：
4.8
作者：
Ladiwala, Ali Reza A.;Litt, Jeffrey;Tessier, Peter M.
通讯作者：
Tessier, Peter M.
影响因子：
3.7
作者：
Cecchelli R;Aday S;Sevin E;Almeida C;Culot M;Dehouck L;Coisne C;Engelhardt B;Dehouck MP;Ferreira L
通讯作者：
Ferreira L
影响因子：
8
作者：
Gokce EH;Korkmaz E;Dellera E;Sandri G;Bonferoni MC;Ozer O
通讯作者：
Ozer O
影响因子：
5.8
作者：
Loureiro, Joana A.;Gomes, Barbara;Pereira, Maria Carmo
通讯作者：
Pereira, Maria Carmo