Presidential symposium : Individualized diagnosis for tailored treatment of breast cancer In vivo single molecular imaging and sentinel node navigation by nanotechnology for molecular targeting drug-delivery systems and tailor-made medicine
Presidential symposium : Individualized diagnosis for tailored treatment of breast cancer In vivo single molecular imaging and sentinel node navigation by nanotechnology for molecular targeting drug-delivery systems and tailor-made medicine
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发表时间：
2008
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通讯作者：
M. Takeda;H. Tada;H. Higuchi;Yoshio Kobayashi;Masaki Kobayashi;Yuu Sakurai;T. Ishida;N. Ohuchi
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作者：
M. Takeda;H. Tada;H. Higuchi;Yoshio Kobayashi;Masaki Kobayashi;Yuu Sakurai;T. Ishida;N. Ohuchi
纳米技术的最新进展在改善人类疾病的预防、诊断和治疗方面具有巨大潜力。在纳米材料和纳米器件的安全性方面，期望医疗应用纳米材料掌握药物动力学和应用于医疗的毒性。我们描述了一代CdSe纳米粒子[量子点（QD）]与单克隆抗HER2抗体（曲妥珠单抗）结合，用于乳腺癌细胞的单分子体内成像。我们建立了一个高分辨率的在体三维显微系统的一种新的成像方法在分子水平上。表达HER2蛋白的癌细胞通过纳米颗粒以亚细胞分辨率在体内可视化，这表明该系统在医疗应用中的未来利用，以改善药物递送系统，以靶向原发性和转移性肿瘤进行定制治疗。我们还描述了前哨淋巴结导航使用荧光纳米粒子的乳腺癌手术的实验模型，这已显示出潜在的替代现有的示踪剂在前哨淋巴结的检测，如果我们选择适当的粒径和波长。通过纳米技术和新的测量技术在癌症成像方面的未来创新将不仅在临床领域而且在基础医学科学方面为医学的发展提供巨大的改进。
The recent advances in nanotechnology have a great potential to improve the prevention, diagnosis, and treatment of human diseases. Nanomaterials for medical applications are expected to grasp pharmacokinetics and the toxicity for application to medical treatment on the aspect of safety of the nanomaterials and nanodevices. We describe a generation of CdSe nanoparticles [quantum dots (QDs)] conjugated with monoclonal anti-HER2 antibody (Trastuzumab), for single molecular in vivo imaging of breast cancer cells. We established a high-resolution in vivo 3D microscopic system for a novel imaging method at the molecular level. The cancer cells expressing HER2 protein were visualized by the nanoparticles in vivo at subcellular resolution, suggesting future utilization of the system in medical applications to improve drug-delivery systems to target the primary and metastatic tumors for made-to-order treatment. We also describe sentinel node navigation using fluorescent nanoparticles for breast cancer surgery in experimental model, which have shown the potential to be an alternative to existing tracers in the detection of the sentinel node if we select the appropriate particle size and wavelength. Future innovation in cancer imaging by nanotechnology and novel measurement technology will provide great improvement, not only in the clinical field but also in basic medical science for the development of medicine.