Innovation of hyaluronic acid-protamine microparticles and their kinetics in vivo.
Innovation of hyaluronic acid-protamine microparticles and their kinetics in vivo.
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透明质酸-鱼精蛋白微粒的创新及其体内动力学。
DOI：
10.1142/s0129083516500054
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
K. Sera. S. Goto
中科院分区：
文献类型：
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作者：
S. Harada ;S. Ehara. K. Ishii ;T. Sato;T. Kamiya ;K. Sera. S. Goto
开发了响应辐射释放抗癌药物的纳米颗粒。同时，还测试了两类药物：（i）它们的体外释放能力;（ii）当它们通过BALB/c小鼠尾静脉注射时，它们在体内的动力学。为了制备颗粒，将透明质酸和鱼精蛋白混合到卡铂溶液中，并在室温下反应30 min。将这些粒子暴露于10戈伊的140 KeV X射线的单次剂量。它们响应于辐射释放卡铂的能力表示为破裂颗粒的百分比，基于颗粒的图像，使用微型PIXE照相机。用PIXE定量分析法测定卡铂的释放量。通过使用微型PIXE相机计数肺部捕获的颗粒数量来评估体内颗粒的动力学。平均粒径为743 ± 34 nm。经照射，59.3 ± 7.23%的颗粒破裂，95.9 ± 2.3%的卡铂从颗粒中释放。与以前的藻酸盐-透明质酸颗粒相比，肺中的捕获颗粒显著减少。
The nanoparticles, which releases anticancer drug with response to radiation, were developed. Also, two categories were tested: (i) their ability to release anticancer drugin vitro; and (ii) their kinetics in the body, when they were injected through tail vein of BALB/c micein vivo. To prepare the particles, hyaluronic acid and protamine were mixed into carboplatin solution, and reacted for 30 min in room temperature. Those particles were exposed to a single dose of 10 Gy of 140 KeV X-ray. Their ability to release carboplatin with response to radiation was expressed as the percentage of ruptured particles, basing on images of particles, using micro PIXE camera. The amount of released carboplatin was measured by quantitative PIXE method. The kinetics of particles in body was assessed by counting the number of particles, which were trapped in lungs, using micro PIXE camera. The mean diameter of particles was 743 ± 34 nm. By irradiation, 59.3 ± 7.23% of particles ruptured, and 95.9 ± 2.3% carboplatin was released from particles. The trapped particles in lungs were significantly reduced, when compared with previous alginate-hyaluronic particles.