Demonstration of array-based analysis for highly multiplexed PCR assays application to detection of IGH@-BCL2 translocations in FFPE follicular lymphoma specimens.
Demonstration of array-based analysis for highly multiplexed PCR assays application to detection of IGH@-BCL2 translocations in FFPE follicular lymphoma specimens.
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演示基于阵列的高度多重 PCR 检测分析应用于检测 FFPE 滤泡性淋巴瘤标本中的 IGH@-BCL2 易位。
DOI：
10.1016/j.jmoldx.2010.11.019
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Burack,WRichard
中科院分区：
文献类型：
--
作者：
Spence,JaniceM;Rothberg,PaulG;Wang,Nancy;Burack,WRichard
We demonstrate an approach that allowed rapid development of a robust assay for the detection of chromosomal translocations. The method includes highly multiplexed PCR with analysis of the PCR products performed by array detection. As proof of principle, we applied this approach to the detection ofIGH@-BCL2translocations in DNA prepared from FFPE specimens. This translocation and specimen type were chosen because of the known difficulties associated with PCR-based detection of this lesion and the additional loss of sensitivity associated with FFPE samples. The multiplex PCR with array detection method detected theIGH@-BCL2translocation in 26 of 36 FFPE follicular lymphoma specimens, whereas the BIOMED-2 assay detected 13 of 36 specimens. This increased sensitivity was the result of both the increased density ofBCL2primers and identification of PCR products by low-density array. The method was specific and allowed mapping of theBCL2break point in all cases. The method detected theIGH@-BCL2lesion when the tumor DNA was diluted more than 1:20 in normal DNA but not when it was diluted more than 1:100. This sensitivity allows detection of diagnostically relevant levels ofIGH@-BCL2but will not detect the rare cells withIGH@-BCL2translocations in healthy individuals.
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DOI：
10.1016/s0091-3057(80)80018-9
发表时间：
1980
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Smith,HE;Lindahl,R
通讯作者：
Lindahl,R
各种组织中人醛脱氢酶同工酶的电泳和生化研究。
DOI：
--
发表时间：
1980
期刊：
Life Science
影响因子：
--
作者：
S. Harada;D. Agarwal;H. Goedde
通讯作者：
H. Goedde
通过聚酰胺层色谱法分离丹磺酰氨基酸。
DOI：
10.1016/0005-2795(67)90078-5
发表时间：
1967
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
K. R. Woods;K. T. Wang
通讯作者：
K. T. Wang
马肝醛脱氢酶 F1 和 F2 同工酶的亚细胞定位。
影响因子：
3.9
作者：
J. Eckfeldt;T. Yonetani
通讯作者：
T. Yonetani
来自狒狒组织的醛脱氢酶、醛氧化酶和黄嘌呤氧化酶：肝脏和大脑中的表型变异性和亚细胞分布。
影响因子：
2.3
作者：
R. Holmes;J. VandeBerg
通讯作者：
J. VandeBerg