The two-component signal system in rice (Oryza sativa L.):: A genome-wide study of cytokinin signal perception and transduction
The two-component signal system in rice (Oryza sativa L.):: A genome-wide study of cytokinin signal perception and transduction
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DOI：
10.1016/j.ygeno.2007.02.001
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发表时间：
2007-06-01
期刊：
影响因子：
4.4
通讯作者：
Wu, Ping
中科院分区：
文献类型：
--
作者：
Du, Liming;Jiao, Fangchan;Wu, Ping
在这份报告中，我们通过对水稻(Oryza sativa L.)的综合计算分析，定义了水稻双组分调控系统的基因。基因组序列数据库。共鉴定出37个基因，包括5个HKS(细胞分裂素反应组氨酸蛋白激酶)(OSHK1-4，OSHKL1)，5个HPS(组氨酸磷酸转移蛋白)(OsHP1-5)，15个A型RRS(反应调节因子)(OsRR1-15)，7个B型RR基因(OsRR16-22)，5个预测假反应调节因子(OsPRR1-5)。描述了水稻、玉米和拟南芥的蛋白质基序组织、基因结构、系统发育分析、染色体定位和比较分析。从水稻中分离到每个基因的全长cdna克隆。在酵母突变体中异源表达的每一种OsHKs都以细胞分裂素依赖的方式赋予组氨酸激酶功能。在表达谱实验中，单个cDNA的非保守区被用作探针。本工作为今后水稻细胞分裂素双组分信号通路的功能解剖奠定了基础。(C)2007 Elsevier Inc.保留所有权利。
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OSHK1-4, OSHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway. (c) 2007 Elsevier Inc. All rights reserved.