In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris
In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris
复制标题
使用毕赤酵母中绿色荧光蛋白融合伴侣体内监测人白细胞介素 2 的细胞内表达
作者：
H. Cha;N. G. Dalal;W. Bentley
通过产生含有绿色荧光蛋白（GFP）和目的产物的融合蛋白，简化了巴斯德毕赤酵母用于蛋白质生产的用途。人白细胞介素-2（hIL-2）被用作模型产物：GFP能够清楚地鉴定融合蛋白的表达，更重要的是，定量hIL-2。虽然GFP融合物用于生物过程监测已在其他宿主中得到证实，但这是其首次在巴斯德毕赤酵母中使用。
The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
影响因子：
3.2
作者：
Monosov, EZ;Wenzel, TJ;Subramani, S
通讯作者：
Subramani, S