Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer
Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer
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DOI：
10.1016/j.cca.2015.01.016
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发表时间：
2015-03-10
影响因子：
5
通讯作者：
Murakami, Masami
中科院分区：
文献类型：
--
作者：
Machida, Tetsuo;Miyashita, Kazuya;Murakami, Masami
背景：脂蛋白脂肪酶（LPL）通过催化甘油三酯的水解，在富含甘油三酯的脂蛋白代谢中发挥核心作用。血清 LPL 的定量可用于诊断血脂紊乱，但尚无可供临床使用的快速测量 LPL 的方法。方法：我们使用乳胶珠固定的抗 LPL 单克隆抗体开发了一种快速、灵敏的乳胶颗粒增强比浊免疫分析 (LTIA) 血清 LPL。该测定在 Hitachi 7700 P 分析仪上进行，并评估其作为与 ELISA 并行定量血清 LPL 浓度的方法的有效性。结果：使用 LTIA 的稀释测试产生了 0.5 至 800 ng/ml 的校准曲线。获得的批内 CV 范围为 2.2-5.5%。在检测含有胆红素-F和C、血红蛋白、甘油三酯和类风湿因子等潜在干扰物质的样本时，未观察到干扰。 LTIA 和 ELISA 之间的强相关性得到证实 (n = 40, r = 0.967, y=0.99x-1.86)。肝素前血清中LPL的正常范围为50-77ng/ml，肝素后血浆中LPL的正常范围分别为354-410ng/ml。结论：LTIA法适用于肝素前血清和肝素后血浆中LPL浓度的定量。该检测方法比ELISA更方便、更快速，非常适合临床常规分析。 (C) 2015 Elsevier B.V. 保留所有权利。
Background: Lipoprotein lipase (LPL) plays a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of serum LPL is useful for diagnosing lipid disorders, but there is no rapid method of measuring LPL for clinical use.Methods: We developed a rapid and sensitive latex particle-enhanced turbidimetric immunoassay (LTIA) serum LPL using latex bead-immobilized anti-LPL monoclonal antibodies. The assay was performed on a Hitachi 7700 P analyzer and evaluated for its validity as a method of quantitating the serum LPL concentration in parallel with ELISA.Results: Dilution tests using LTIA produced a calibration curve from 0.5 to 800 ng/ml. Within-run CV was obtained in the range of 2.2-5.5%. No interference was observed in the testing of specimens containing potentially interfering substances such as bilirubin-F and C, hemoglobin, triglycerides and rheumatoid factor. A strong correlation between LTIA and ELISA was confirmed (n = 40, r = 0.967, y=0.99x-1.86). The normal range of LPL in pre-heparin serum was 50-77 ng/ml and in post-heparin plasma 354-410 ng/ml, respectively.Conclusion: The LTIA assay is applicable in quantitating the concentration of LPL in both pre-heparin serum and post-heparin plasma. This assay is more convenient and faster than ELISA and highly suitable for clinical routine analysis. (C) 2015 Elsevier B.V. All rights reserved.