Clog and Release, and Reverse Motions of DNA in a Nanopore
Clog and Release, and Reverse Motions of DNA in a Nanopore
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DOI：
10.3390/polym11010084
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发表时间：
2019-01-01
期刊：
影响因子：
5
通讯作者：
Mitsui, Toshiyuki
中科院分区：
文献类型：
--
作者：
Kubota, Tomoya;Lloyd, Kento;Mitsui, Toshiyuki
用荧光显微镜实验观察和研究了不同长度的圆形和线形DNA分子在直径为100 nm和200 nm的纳米孔附近的运动。DNA分子通过纳米孔的移动被称为移位，主要是由孔附近和孔内的电场驱动的。我们发现DNA分子对纳米孔有显著的堵塞作用，尤其是环状DNA和线形T4DNA(165.65 kbp)。在这里，DNA堵塞事件的概率取决于DNA的长度和形状，直线或圆形。此外，还观察到了两种不同的DNA运动：线性T4DNA的堵塞和释放，以及环形DNA在孔入口处的反向运动，之后两个分子都离开了孔。进行了基于有限元方法的数值模拟。结果表明，孔径为100-200 nm的DNA分子受到反向流体力学流动的强烈影响，而大体积DNA构型则进一步增强了这种影响。
Motions of circular and linear DNA molecules of various lengths near a nanopore of 100 or 200 nm diameter were experimentally observed and investigated by fluorescence microscopy. The movement of DNA molecules through nanopores, known as translocation, is mainly driven by electric fields near and inside the pores. We found significant clogging of nanopores by DNA molecules, particularly by circular DNA and linear T4 DNA (165.65 kbp). Here, the probabilities of DNA clogging events, depending on the DNA length and shapelinear or circularwere determined. Furthermore, two distinct DNA motions were observed: clog and release by linear T4 DNA, and a reverse direction motion at the pore entrance by circular DNA, after which both molecules moved away from the pore. Finite element method-based numerical simulations were performed. The results indicated that DNA molecules with pores 100-200 nm in diameter were strongly influenced by opposing hydrodynamic streaming flow, which was further enhanced by bulky DNA configurations.