The adenosine triphosphatase activity of acto-heavy meromyosin. A kinetic analysis of actin activation.
The adenosine triphosphatase activity of acto-heavy meromyosin. A kinetic analysis of actin activation.
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重肌球蛋白的腺苷三磷酸酶活性。
DOI：
10.1021/bi00844a035
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发表时间：
1968
期刊：
影响因子：
2.9
通讯作者：
C. Moos
中科院分区：
文献类型：
--
作者：
E. Eisenberg;C. Moos
方法肌球蛋白和肌球蛋白的制备均采用本实验室已有的方法(Eisenberg and Moos，1967)，不同之处在于制备肌球蛋白时用胰酶消化5min而不是4min。ATPase也像以前一样被测量，使用自动pH-Stat，样品在25。在所有情况下，反应都是由添加重肌球蛋白引发的，并且在整个反应过程中速度总是恒定的，正如我们以前报道的在低离子强度下的重肌球蛋白(Eisenberg和Moos，1967)。
MethodsActin and heavy meromyosin were prepared by the methods previously used in our laboratory (Eisenberg and Moos, 1967) except that in the preparation of heavy meromyosin, the myosin was digested with trypsin for 5 rather than 4 min. The ATPase was also measured as previously, using an automatic pH-Stat with the sample at 25. In all cases the reaction was initiated by the addition of heavy meromyosin and the rate was always constant throughout the course of the reaction, as we have previously reported for acto-heavy meromyosin at low ionic strength (Eisenberg and Moos, 1967).