Secretion from corticotropes after avidin-fluorescein stains for biotinylated ligands (CRF or AVP).
Secretion from corticotropes after avidin-fluorescein stains for biotinylated ligands (CRF or AVP).
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生物素化配体（CRF 或 AVP）亲和素-荧光素染色后促肾上腺皮质激素的分泌。
DOI：
10.1152/ajpendo.1987.252.3.e347
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. Marchetti
中科院分区：
文献类型：
--
作者：
G. Childs;G. Unabia;J. Burke;C. Marchetti
用生物素标记的促肾上腺皮质激素释放因子（1-Bio-CRF）或精氨酸抗利尿素（Bio-AVP）和亲和素荧光素（细胞分级）鉴定存活的垂体靶细胞。如果加入非生物素化配体与相应的生物素化配体竞争特定的结合位点，则染色率从8-10%降至3%以下。然而，AVP没有降低1-Bio-CRF的染色，也没有降低Bio-AVP的染色。在染色过程中加入1-Bio-CRF或Bio-AVP时，中膜促肾上腺皮质激素（ACTH）水平增加4-18倍。染色1小时后，细胞分泌的ACTH水平与染色前的基础水平相同。当在这一小时后用CRF或AVP刺激时，细胞再次反应，与染色时所见的增加没有什么不同。染色细胞群的实验也表明，细胞在染色后24小时具有完全的反应性。ACTH的反向溶血斑块试验，用于研究单个染色细胞的分泌，显示大约80%的斑块含有染色细胞，斑块的数量和面积与未染色的人群相似。这些数据表明，在暴露于低剂量生物素化配体（50-500 pM）时，促肾上腺皮质激素不会慢性脱敏。
Viable pituitary target cells were identified with biotin-labeled corticotropin-releasing factor (1-Bio-CRF) or arginine vasopressin (Bio-AVP) and avidin-fluorescein (cell sorter grade). Stain was reduced from 8-10% of pituitary cells to less than 3% if nonbiotinylated ligands were added to compete with their respective biotinylated ligand for specific binding sites. However, AVP did not decrease the stain for 1-Bio-CRF nor did CRF decrease the Bio-AVP stain. Media adrenocorticotropin (ACTH) levels increased 4-18 times when 1-Bio-CRF or Bio-AVP were added during the staining process. One hour after staining, the cells secreted ACTH at levels identical to prestain basal levels. When challenged with CRF or AVP after this hour, the cells responded again with increases not different from those seen during staining. Tests of stained cell populations also showed that the cells were fully responsive 24 h after staining. The reverse hemolytic plaque assay for ACTH, which was used to study secretion from individual stained cells, showed that approximately 80% of plaques contained a stained cell and that plaque numbers and areas were like those of unstained populations. These data show that corticotropes are not chronically desensitized during exposure to low doses of biotinylated ligands (50-500 pM).
培养物中雄性和雌性垂体细胞中催乳素和生长激素的计数：支持大鼠中催乳素亚群的证据。
影响因子：
4.8
作者：
Leong,DA;Lau,SK;Sinha,YN;Kaiser,DL;Thorner,MO
通讯作者：
Thorner,MO
新生雄性大鼠应激无反应期前叶促肾上腺皮质激素在促肾上腺皮质激素和促性腺激素亚群中的储存。
影响因子：
4.8
作者：
Childs,GV;Ellison,DG;Ramaley,JA
通讯作者：
Ramaley,JA
用抗生物素蛋白-生物素-过氧化物酶复合物将生物素化促性腺激素释放激素定位在垂体单层细胞上。
DOI：
10.1177/31.12.6195217
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Childs,GV;Naor,Z;Hazum,E;Tibolt,R;Westlund,KN;Hancock,MB
通讯作者：
Hancock,MB