A CONVENIENT SPECTROPHOTOMETRIC ASSAY FOR PHOSPHODIESTERASES USING DINUCLEOSIDE-MONOPHOSPHATES AS SUBSTRATES
A CONVENIENT SPECTROPHOTOMETRIC ASSAY FOR PHOSPHODIESTERASES USING DINUCLEOSIDE-MONOPHOSPHATES AS SUBSTRATES
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DOI：
10.1111/j.1432-1033.1969.tb00511.x
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发表时间：
1969-01-01
期刊：
影响因子：
--
通讯作者：
FELICIOL.RA
中科院分区：
文献类型：
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作者：
IPATA, PL;FELICIOL.RA
本文介绍了一种基于分光光度法测定腺苷酰-（3′-5′）-腺苷、鸟苷酰-（3 ′-5 ′）-腺苷和腺苷酰-（3 ′-5 ′）-鸟苷释放腺苷的脾-蛇毒磷酸二酯酶偶联光酶试验。以二核苷单磷酸为底物，加入过量的腺苷脱氨酶，在265 μ m处吸光度下降，腺苷从底物中释放出来。因为腺苷仅通过产生磷酸二酯酶的3 ′-单核苷酸从GpA释放，并且仅通过产生磷酸二酯酶的5′-单核苷酸从ApG释放。
A coupled optical enzyme test for spleen and snake venom phosphodiesterase is described, that is based on the spectrophotometric determination of adenosine released from adenylyl‐(3′‐5′)‐adenosine, guanylyl‐(3′‐5′)‐adenosine and adenylyl‐(3′‐5′)‐guanosine. The liberation of adenosine from the dinucleoside monophosphates used as substrates is followed from the decrease in absorbance at 265 mμ, after addition of an excess of adenosine deaminase.The method proposed allows the detection of the specificity of a phosphodiesterase,i. e.whether a 3′‐mononucleotide or a 5′‐mononucleotide is released, since adenosine is liberated from GpA only by 3′‐mononucleotides producing phosphodiesterases, and from ApG only by 5′‐mononucleotides producing phosphodiesterases.