AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MONITORING RODENT COLONIES FOR PASTEURELLA-PNEUMOTROPICA ANTIBODIES
AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MONITORING RODENT COLONIES FOR PASTEURELLA-PNEUMOTROPICA ANTIBODIES
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DOI：
10.1258/002367795781088306
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发表时间：
1995-07-01
影响因子：
2.4
通讯作者：
BAKKER, RGH
中科院分区：
文献类型：
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作者：
BOOT, R;THUIS, HCW;BAKKER, RGH
建立了检测大鼠、小鼠、仓鼠和马斯泰杆菌血清中嗜肺巴氏杆菌抗体的酶联免疫吸附试验(ELISA)。小鼠和大鼠嗜肺假单胞菌表现出交叉反应。用嗜肺假单胞菌NCTC 8284建立的酶联免疫吸附试验在培养嗜肺假单胞菌、嗜血杆菌和/或放线杆菌的啮齿类动物中检测到比选择性培养更多的感染动物。嗜肺假单胞菌NCTC8284与嗜血杆菌和放线杆菌的交叉反应未见报道。
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.