Sequential delivery of vascular endothelial growth factor and sphingosine 1-phosphate for angiogenesis.
Sequential delivery of vascular endothelial growth factor and sphingosine 1-phosphate for angiogenesis.
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DOI：
10.1016/j.biomaterials.2010.07.010
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发表时间：
2010-10
期刊：
影响因子：
14
通讯作者：
Little SR
中科院分区：
文献类型：
--
作者：
Tengood JE;Kovach KM;Vescovi PE;Russell AJ;Little SR
血管生成是一系列有组织的事件，从血管不稳定开始，随后是内皮细胞重组，并以血管成熟结束。血管内皮生长因子（VEGF）有助于血管通透性和内皮细胞募集，而1-磷酸鞘氨醇（S1 P）刺激血管稳定性。因此，VEGF可以抑制血管稳定，而S1 P可以抑制内皮细胞募集。出于这个原因，我们创建了一个新的外部调节的交付模式，不仅允许生物活性因子的持续释放，而且还暂时分离的生长因子的交付。使用该模型，首先在体外证实了因子的顺序递送，相关内皮细胞以剂量依赖性方式响应。此外，使用改良的鼠基质胶栓模型，很明显，其中VEGF呈递与S1 P呈递暂时分离的递送策略不仅导致内皮细胞的更大募集，而且还导致相关血管的更高成熟指数。
Angiogenesis is an organized series of events, beginning with vessel destabilization, followed by endothelial cell re-organization, and ending with vessel maturation. Vascular endothelial growth factor (VEGF) aids in vascular permeability and endothelial cell recruitment while sphingosine 1-phosphate (S1P) stimulates vascular stability. Accordingly, VEGF may inhibit vessel stabilization while S1P may inhibit endothelial cell recruitment. For this reason, we created a new externally-regulated delivery model that not only permits sustained release of bioactive factors, but also temporal separation of the delivery of growth factors. Using this model, sequential delivery of factors was first confirmed in vitro with associated endothelial cells responding in a dose dependent manner. Furthermore, using a modified murine Matrigel plug model, it is apparent that delivery strategies where VEGF presentation is temporally separated from S1P presentation not only led to greater recruitment of endothelial cells, but also higher maturation index of associated vessels.
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影响因子：
--
作者：
Huang, Zhinong;Nelson, Ehren Robert;Goodman, Stuart B.
通讯作者：
Goodman, Stuart B.
影响因子：
4
作者：
Kano, MR;Morishita, Y;Miyazawa, K
通讯作者：
Miyazawa, K
缺乏周细胞会导致内皮增生和异常血管形态发生。
影响因子：
7.8
作者：
Hellstrom, M;Gerhardt, H;Kalen, M;Li, X;Eriksson, U;Wolburg, H;Betsholtz, C
通讯作者：
Betsholtz, C
影响因子：
64.8
作者：
Greenberg, Joshua I.;Shields, David J.;Barillas, Samuel G.;Acevedo, Lisette M.;Murphy, Eric;Huang, Jianhua;Scheppke, Lea;Stockmann, Christian;Johnson, Randall S.;Angle, Niren;Cheresh, David A.
通讯作者：
Cheresh, David A.
DOI：
10.1006/bbrc.1999.1586
发表时间：
1999-11-02
影响因子：
3.1
作者：
Lee, OH;Kim, YM;Kwon, YG
通讯作者：
Kwon, YG