Mediation of apoptosis and proliferation of human embryonic stem cells by sphingosine-1-phosphate
Mediation of apoptosis and proliferation of human embryonic stem cells by sphingosine-1-phosphate
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DOI：
10.1089/scd.2006.15.789
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发表时间：
2006-12-01
影响因子：
4
通讯作者：
Moore, Harry
中科院分区：
文献类型：
--
作者：
Inniss, Katie;Moore, Harry
人类胚胎干细胞(HES)通过自我更新的过程进行复制，同时保持其多能性。这一过程可能由几种不同的途径调节，人们对此知之甚少。在本研究中，鞘氨醇-1-磷酸(S1P)受体定位于几个细胞系(SHEF 1-6)的HES细胞上，并通过RT-PCR和Western blotting证实了其存在。干细胞标记物Tra-1-60双重染色显示多潜能细胞上存在S1P受体。添加20 mU M S1P的培养基能显著降低各系细胞的凋亡率。S1P处理也被发现在统计学上增加了细胞培养中的增殖水平。所有的SHEF细胞系对S1P处理的反应方式相似；然而，细胞系之间的微小差异是明显的。S1P指导多种细胞类型的细胞命运决定。在这里，我们展示了S1P通过减少凋亡和促进增殖在HES细胞存活中的作用。
Human embryonic stem (hES) cells replicate by the process of self-renewal while maintaining their pluripotency. This process may be regulated by several different pathways and is poorly understood. In this study, sphingosine-1-phosphate (S1P) receptors were localized on hES cells of several cell lines (Shef 1-6), and their presence was verified by RT (reverse transcriptase)-PCR and western blotting. Dual staining with the stem cell marker Tra-1-60 revealed the presence of S1P receptors on pluripotential cells. Medium supplemented with 20 mu M S1P significantly reduced the level of apoptosis in cultures of each of the Shef lines. S1P treatment was also found to statistically increase the level of proliferation in cell cultures. All Shef lines responded to S1P treatment in a similar manner; however, minor differences between cell lines were apparent. S1P directs cell fate decisions of many cell types. Here we demonstrate a role for S1P in hES cell survival by reducing apoptosis and increasing proliferation.