DEVELOPMENTAL-CHANGES IN THE RESPIRATORY-CHAIN OF ASCARIS MITOCHONDRIA
DEVELOPMENTAL-CHANGES IN THE RESPIRATORY-CHAIN OF ASCARIS MITOCHONDRIA
复制标题
DOI：
10.1016/0005-2728(93)90190-q
复制
发表时间：
1993-02-08
期刊：
影响因子：
--
通讯作者：
AOKI, T
中科院分区：
文献类型：
--
作者：
TAKAMIYA, S;KITA, K;AOKI, T
蛔虫幼虫呼吸链，特别是复合物II（琥珀酸-泛醌氧化还原酶），在分离的线粒体进行了表征。低温差光谱表明，存在底物可还原的细胞色素aa 3的CoMPleX IV，c + c1和B的复合物III（泛喹啉-细胞色素c氧化还原酶）的线粒体从第二阶段的幼虫（L2线粒体）。通过高效液相色谱法的醌分析表明，不像成人线粒体，其中只含有玫瑰醌-9，L2线粒体含有泛醌-9作为主要成分。L2线粒体中的复合物II在动力学上不同于成人线粒体中的复合物II。个体氧化还原酶活性包括琥珀酸氧化酶和延胡索酸还原酶，测定线粒体从L2幼虫，从幼虫培养到后期阶段，并从成年线虫。L2线粒体的细胞色素c氧化酶比活性最高，表明L2幼虫具有最好氧的呼吸链的研究阶段。在L2和培养的幼虫线粒体中复合物II的Cyb（S）亚基对抗成虫Cyb（S）抗体表现出不同的反应性。两者合计，这些结果表明，复杂II的幼虫是不同的，从它的成年对应。与线粒体生物发生的这种变化平行，在蛔虫线虫的发育过程中发生醌类的生物合成转化。
The Ascaris larval respiratory chain, particularly complex II (succinate-ubiquinone oxidoreductase), was characterize in isolate mitochondria. Low-temperature difference spectra showed the presence of substrate-reducible cytochromes aa3 of CoMPleX IV, c + c1 and b of complex III (ubiquinol-cytochrome c oxidoreductase) in mitochondria from second-stage larvae (L2 mitochondria). Quinone analysis by high-performance liquid chromatography showed that, unlike adult mitochondria, which contain only rhodoquinone-9, L2 mitochondria contain ubiquinone-9 as a major component. Complex II in L2 mitochondria was kinetically different from that in adult mitochondria. The individual oxidoreductase activities comprising succinate oxidase, and fumarate reductase were determined in mitochondria from L2 larvae, from larvae cultured to later stages, and from adult nematodes. The L2 mitochondria exhibited the highest specific activity of cytochrome c oxidase, indicating that L2 larvae have the most aerobic respiratory chain among the stages studied. The Cyb(S) subunit of complex II in L2 and cultured-larvae mitochondria exhibited different reactivities against anti-adult Cyb(S) antibodies. Taken together, these results indicate that the complex II of larvae is different from its adult counterpart. In parallel with this change in mitochondrial biogenesis, biosynthetic conversion of quinones occurs during development in Ascaris nematodes.