VASCULAR SMOOTH-MUSCLE CELLS DIFFER FROM OTHER SMOOTH-MUSCLE CELLS - PREDOMINANCE OF VIMENTIN FILAMENTS AND A SPECIFIC ALPHA-TYPE ACTIN
VASCULAR SMOOTH-MUSCLE CELLS DIFFER FROM OTHER SMOOTH-MUSCLE CELLS - PREDOMINANCE OF VIMENTIN FILAMENTS AND A SPECIFIC ALPHA-TYPE ACTIN
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DOI：
10.1073/pnas.78.1.298
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
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作者：
GABBIANI, G;SCHMID, E;FRANKE, WW
消化道、呼吸道和泌尿生殖道的平滑肌细胞含有结蛋白作为其主要的（如果不是唯一的）中等大小的丝成分，并且还显示出γ型平滑肌肌动蛋白的优势。通过细胞蛋白的一维和二维凝胶电泳以及使用针对不同中等尺寸丝蛋白的抗体的免疫荧光显微镜检查来自不同哺乳动物（人、牛、猪、兔和大鼠）的不同血管（例如主动脉、小动脉、小动脉、小静脉和腔静脉）的平滑肌组织。血管平滑肌细胞的中等大小的丝含有大量的波形蛋白和少量（如果有的话）结蛋白。在凝胶电泳上，血管平滑肌波形蛋白显示为表观 pI [等电点] 值 5.30 和 5.29 的 2 个等电变体，显示出一系列特征性的蛋白水解片段，是主要的细胞蛋白之一。波形蛋白在体内的平滑肌细胞中得到证实。血管平滑肌细胞的特征在于平滑肌特异性α型肌动蛋白占主导地位，而γ型平滑肌肌动蛋白作为次要成分存在。血管平滑肌细胞的中间丝和肌动蛋白组成显然反映了有别于其他平滑肌细胞的分化途径，可能与血管的特殊功能和病理紊乱有关。
Smooth muscle cells of the digestive, respiratory and urogenital tracts contain desmin as their major, if not exclusive, intermediate-sized filament constituent and also show a predominance of .gamma.-type smooth muscle actin. Smooth muscle tissue of different blood vessels (e.g., aorta, small arteries, arterioles, venules, and vena cava) from various mammals (man, cow, pig, rabbit and rat) was examined by 1- and 2-dimensional gel electrophoresis of cell proteins and by immunofluorescence microscopy using antibodies to different intermediate-size filament proteins. Intermediate-sized filaments of vascular smooth muscle cells contain abundant amounts of vimentin and little, if any, desmin. On gel electrophoresis vascular smooth muscle vimentin appears as 2 isoelectric variants of apparent pI [isoelectric point] values of 5.30 and 5.29, shows the characteristic series of proteolytic fragments, and is one of the major cell proteins. Vimentin was demonstrated in a smooth muscle cell present in the body. Vascular smooth muscle cells are distinguished by the predominance of a smooth muscle-specific .alpha.-type actin, whereas .gamma.-type smooth muscle actin is present as a minor component. The intermediate filament and actin composition of vascular smooth muscle cells apparently reflects a differentiation pathway separate from that of other smooth muscle cells and may be related to special functions and pathological disorders of blood vessels.