Primer-DNA formation during simian virus 40 DNA replication in vitro.
Primer-DNA formation during simian virus 40 DNA replication in vitro.
复制标题
猿猴病毒 40 DNA 体外复制过程中引物 DNA 的形成。
DOI：
10.1128/mcb.13.5.2882-2890.1993
复制
发表时间：
1993
影响因子：
5.3
通讯作者：
Bullock,PA
中科院分区：
文献类型：
--
作者：
Denis,D;Bullock,PA
Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (∼ 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. Initial experiments indicated that T antigen and the polymerase alpha-primase complex are required to form primer-DNA. Proliferating cell nuclear antigen, and presumably proliferating cell nuclear antigen-dependent polymerases, is not needed to form this species. Herein, we present an investigation of the stages at which primer-DNA functions during SV40 DNA replication in vitro. Hybridization studies indicate that primer-DNA is initially formed in the origin region and is subsequently synthesized in regions distal to the origin. At all time points, primer-DNA is synthesized from templates for lagging-strand DNA replication. These studies indicate that primer-DNA functions during both initiation and elongation stages of SV40 DNA synthesis. Results of additional experiments suggesting a precursor-product relationship between formation of primer-DNA and Okazaki fragments are presented.
