Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences
Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences
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DOI：
10.1021/acsinfecdis.8b00356
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发表时间：
2019-06-01
影响因子：
5.3
通讯作者：
Jarrold, Martin F.
中科院分区：
文献类型：
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作者：
Dunbar, Carmen A.;Rayaprolu, Vamseedhar;Jarrold, Martin F.
辛德比斯病毒(SINV)是一种有包膜的单链RNA病毒，通过蚊子传播给多种脊椎动物宿主。脊椎动物幼年仓鼠肾脏(BHK)细胞产生的SINV的传染性比蚊子(C6/36)细胞产生的SINV低一个数量级以上。造成这种差异的原因人们知之甚少。本研究用电荷检测质谱仪测定了从BHK和C6/36细胞中分离出的完整SINV颗粒的质量。测量的质量有很大的不同：BHK来源的SINV为52.88 mda，C6/36来源的mda为50.69 mda。使用几种基于质谱学方法和生物物理方法的进一步分析表明，BHK来源的SINV比C6/36来源的SINV具有更高的质量，这是因为在脂质双层中，含有较高比例的长链脂肪酸的脂类。脂类组成的不同会影响脂类双层的结构。因此，病毒生命周期的多个阶段可能会受到影响，包括组装和萌芽、传播过程中的颗粒稳定性和融合事件，所有这些都可能导致传染性的差异。
Sindbis virus (SINV) is an enveloped, single-stranded RNA virus, which is transmitted via mosquitos to a wide range of vertebrate hosts. SINV produced by vertebrate, baby hamster kidney (BHK) cells is more than an order of magnitude less infectious than SINV produced from mosquito (C6/36) cells. The cause of this difference is poorly understood. In this study, charge detection mass spectrometry was used to determine the masses of intact SINV particles isolated from BHK and C6/36 cells. The measured masses are substantially different: 52.88 MDa for BHK derived SINV and 50.69 MDa for C6/36 derived. Further analysis using several mass spectrometry-based methods and biophysical approaches indicates that BHK derived SINV has a substantially higher mass than C6/36 derived because in the lipid bilayer, there is a higher portion of lipids containing long chain fatty acids. The difference in lipid composition could influence the organization of the lipid bilayer. As a result, multiple stages of the viral lifecycle may be affected including assembly and budding, particle stability during transmission, and fusion events, all of which could contribute to the differences in infectivity.