KIAA1718 is a histone demethylase that erases repressive histone methyl marks
KIAA1718 is a histone demethylase that erases repressive histone methyl marks
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DOI：
10.1111/j.1365-2443.2010.01424.x
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发表时间：
2010-08-01
期刊：
影响因子：
2.1
通讯作者：
Kato, Shigeaki
中科院分区：
文献类型：
--
作者：
Yokoyama, Atsushi;Okuno, Yosuke;Kato, Shigeaki
组蛋白赖氨酸残基的甲基化状态被认为是控制转录调控的重要表观遗传标记。许多含有 jumonji C (JmjC) 结构域的组蛋白去甲基酶已被识别；然而，它们的特性仍有待研究。在这里，我们发现 KIAA1718（PHF2/PHF8 亚家族成员）具有对 H3K9 和 H3K27（转录抑制组蛋白标记）特异的组蛋白去甲基化酶活性。 KIAA1718 相互作用物的生化纯化表明 KIAA1718 与多种因子形成复合物，其中包括转录共激活因子 KAP1。与这些发现一致，KIAA1718 在染色质环境中显示出转录激活功能。因此，我们的研究将 KIAA1718 鉴定为抑制甲基标记的组蛋白去甲基化酶，并表明它参与转录激活。
The methylation states of histone lysine residues are regarded as significant epigenetic marks governing transcriptional regulation. A number of histone demethylases containing a jumonji C (JmjC) domain have been recognized; however, their properties remain to be investigated. Here, we show that KIAA1718, a PHF2/PHF8 subfamily member, possesses histone demethylase activity specific for H3K9 and H3K27, transcriptionally repressive histone marks. Biochemical purification of the KIAA1718 interactants reveals that KIAA1718 forms complexes with several factors including KAP1, a transcriptional co-activator. Consistent with these findings, KIAA1718 shows a transcriptional activation function in the chromatin context. Thus, our study identifies KIAA1718 as a histone demethylase for repressive methyl marks and shows that it is involved in transcriptional activation.