Efficacy of tear eosinophil cationic protein level measurement using filter paper for diagnosing allergic conjunctival disorders
Efficacy of tear eosinophil cationic protein level measurement using filter paper for diagnosing allergic conjunctival disorders
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DOI：
10.1016/s0021-5155(02)00630-5
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Kato, H
中科院分区：
文献类型：
--
作者：
Shoji, J;Kitazawa, M;Kato, H
目的：我们调查的有效性的泪液采样方法，使用滤纸，以评估嗜酸性粒细胞阳离子蛋白（ECP）的水平在过敏性disorders.Methods患者：受试者是一个过敏性组，包括过敏性结膜炎，春季角结膜炎，或特应性角结膜炎，Sjogren组，包括继发性Sjogren综合征患者，和对照组，包括健康志愿者。使用Schirmer方法I对眼泪取样，并将样品从滤纸上在50穆尔洗脱溶液中洗脱，所述洗脱溶液包含含有0.5 M NaCl + 0.1%吐温20的磷酸盐缓冲溶液。结果：过敏组泪液ECP水平明显高于其他两组（P <0.01），而对照组则明显低于过敏组（P <0.001）。此外，过敏组各过敏性疾病亚组的泪液ECP水平均显著高于对照组。二、结论：这种测定泪液ECP浓度的方法不仅可用于诊断过敏性结膜疾病，而且可用于评估其临床分期。Jpn J Ophthalmol 2003;47：64-68（C）2003日本眼科学会。
Purpose: We investigated the efficacy of the tear sampling method using a filter paper to evaluate eosinophil cationic protein (ECP) levels in patients with allergic disorders.Methods: Subjects were an allergic group comprising patients with allergic conjunctivitis, vernal keratoconjunctivitis, or atopic keratoconjunctivitis, a Sjogren group comprising patients with secondary Sjogren syndrome, and a control group comprising healthy volunteers. Tears were sampled using the Schirmer Method I and the sample was eluted from the filter paper in 50 muL of elution solution containing phosphate buffer solution with 0.5 M NaCl + 0.1% Tween 20. Then the ECP concentration in the elution sample was determined by the enzyme-linked immunosorbent assay method.Results: Tear ECP level in the allergic group was significantly higher than the levels in the other two groups (P < .01 for the Sjogren group and P < .001 for the control). Furthermore, the tear ECP level of each allergic disease subgroup in the allergic group was significantly higher than that in the control group. .Conclusions: This method of determining tear ECP concentration is useful not only to diagnose allergic conjunctival disorders but also to evaluate their clinical stages. Jpn J Ophthalmol 2003;47:64-68 (C) 2003 Japanese Ophthalmological Society.