MODIFICATION OF LOWRY PROCEDURE TO SIMPLIFY PROTEIN DETERMINATION IN MEMBRANE AND LIPOPROTEIN SAMPLES
MODIFICATION OF LOWRY PROCEDURE TO SIMPLIFY PROTEIN DETERMINATION IN MEMBRANE AND LIPOPROTEIN SAMPLES
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DOI：
10.1016/0003-2697(78)90586-9
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
TOLBERT, NE
中科院分区：
文献类型：
--
作者：
MARKWELL, MAK;HAAS, SM;TOLBERT, NE
通过在碱性试剂中加入十二烷基硫酸钠和增加酒石酸铜试剂的量，对原Lowry法测定蛋白质进行了改进。这些改变允许该方法用于[大鼠肝]膜和脂蛋白制剂，而无需事先溶解或脂质提取，并与含有200 mM蔗糖或2.5 mM EDTA的样品一起使用。
The original Lowry method of protein determination was modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent. These alterations allowed the method to be used with [rat liver] membrane and lipoprotein preparations without prior solubilization or lipid extraction and with samples containing 200 mM sucrose or 2.5 mM EDTA.