COMPARATIVE HISTOPATHOLOGY IN BALB/C MICE INFECTED WITH VIRULENT AND ATTENUATED STRAINS OF BRUCELLA-ABORTUS
COMPARATIVE HISTOPATHOLOGY IN BALB/C MICE INFECTED WITH VIRULENT AND ATTENUATED STRAINS OF BRUCELLA-ABORTUS
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DOI：
10.1016/0165-2427(90)90065-z
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发表时间：
1990-10-01
影响因子：
1.8
通讯作者：
WINTER, AJ
中科院分区：
文献类型：
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作者：
ENRIGHT, FM;ARAYA, LN;WINTER, AJ
牛种布氏杆菌强毒株2308（S-2308）和弱毒株19（S-19）在BALB/c小鼠体内的感染过程有明显差异。而S-19在感染后2周（p.i.）开始以指数速率消除，菌株2308在感染后的前6周期间呈现稳定状态或平台。然后在超过6个月的时间内非常缓慢地被消除。在这里，我们比较了感染两种B菌株的小鼠脾脏和肝脏中炎症反应的启动和维持。前6周p.i.流产肝脏的组织学变化对两种菌株的反应相似，其特征在于小肉芽肿的发展和多形核白细胞（PMN）和单核细胞的流入。在感染后第1周和第2周，脾脏中的组织反应相似。给药后3周此后，S-2308感染小鼠的局灶性肉芽肿反应超过了S-19感染小鼠。在S-19感染的脾脏中，非特异性酯酶（NSE）阳性单核白细胞的数量在感染后3周增加。并保持高位。在S-2308感染的小鼠中，NSE阳性细胞没有类似的增加，数量显著降低。在4周p.i. S-19感染的脾成熟中性粒细胞的流入和髓外造血的强度显著更大。在感染后前3周，在两种感染中均观察到动脉周围淋巴组织严重耗竭。然而，S-19感染脾脏中淋巴鞘的再增殖在感染后4周显著增加。并在接下来的两周内以显著更高的水平继续增加。本研究证明了脾脏炎症反应的定量差异，其与S-19的更快速消除存在时间相关性。基于菌株2308对免疫血清保护作用的敏感性较低，假设2种菌株显示的不同感染和炎症模式可能与抗体调理的S-19和S-2308在活化巨噬细胞中存活的能力差异有关。
The course of infection in BALB/c mice of virulent Brucella abortus strain 2308 (S-2308) and attentuated strain 19 (S-19) varies markedly. Whereas S-19 is eliminated at an exponential rate beginning at 2 weeks post infection (p.i.), strain 2308 assumes a steady state or plateau during the first 6 weeks p.i. and thereafter is eliminated very slowly over a period exceeding 6 months. Here we compared the initiation and maintenance of inflammatory reactions in spleens and livers of mice infected with either of the two strains of B. abortus for the first 6 weeks p.i. Histological changes in the liver were similar in response to either strain and were characterized by the development of small granulomas and an influx of polymorphonuclear leukocytes (PMN) and monocytes. Tissue reactions in the spleen were similar at weeks 1 and 2 p.i. At 3 weeks p.i. and thereafter, focal granulomatous responses in S-2308-infected mice exceeded those in mice infected with S-19. Numbers of nonspecific esterase (NSE) positive mononuclear leukocytes in S-19-infected spleens had increased by 3 weeks p.i. and remained elevated. No comparable increase in NSE positive cells occurred in mice infected with S-2308, and numbers were significantly lower. At 4 weeks p.i. the influx of mature neutrophils and the intensity of extramedullary hematopoiesis were significantly greater in S-19-infected spleens. A profound depletion of periarteriolar lymphoid tissue was noted in both infections for the first 3 weeks p.i. However, repopulation of lymphoid sheaths in S-19-infected spleens became significantly greater by 4 weeks p.i. and continued to increase at significantly higher levels during the next 2 weeks. This study demonstrates quantitative differences in splenic inflammatory responses which are temporally related to the more rapid elimination of S-19. Based upon the lower susceptibility of strain 2308 to the protective effects of immune serum it is hypothesized that the different patterns of infection and inflammation displayed by the 2 strains may relate to the differential capacities of antibody opsonized S-19 and S-2308 to survive in activated macrophages.