INTRA-CELLULAR LIPIDS OF CARTILAGE
INTRA-CELLULAR LIPIDS OF CARTILAGE
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DOI：
10.1136/ard.24.2.123
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发表时间：
1965-01-01
影响因子：
27.4
通讯作者：
MEACHIM, G
中科院分区：
文献类型：
--
作者：
COLLINS, DH;GHADIALLY, FN;MEACHIM, G
细胞内脂质的形态和分布已被研究在透明软骨从仓鼠，兔，大鼠和人。在油红-0染色的冷冻切片的光学显微镜下，脂质被认为是在形式范围从一个单一的小或细滴到许多这些或一个大滴，可以比细胞核大得多。电子显微镜显示了相应范围的细胞内模式，并表明这些都可以发生在表面健康的软骨细胞。在所研究的动物中，种属对细胞内脂质模式的影响不如年龄、样本部位以及软骨细胞相对于关节面或软骨膜的位置的影响显著。通常，在成年人的软骨中，比在婴儿期和生长早期，细胞内脂质更多。在2天大和6个月大的仓鼠之间、人类婴儿和成年人之间以及800 g和2 - 3 kg的兔子之间，已经注意到年龄差异。在成人的透明软骨中，软骨细胞几乎都含有一个或多个脂滴。在成人肋软骨和支气管软骨的中央块中，细胞可以显示比细胞核更大的液滴，它们可以移位到细胞的外围。相比之下，在软骨膜下或关节软骨细胞中没有遇到这种大小的脂滴。
The morphology and distribution of intracellular lipid has been studied in hyaline cartilage from the hamster, rabbit, rat, and man. On light microscopy of oil red-0 stained frozen sections, lipid is seen in forms ranging from a single small or fine drop to many of these or to a large drop which can be much bigger than the nucleus. Electron microscopy shows a corresponding range of intracellular patterns, and indicates that each of these can occur in apparently healthy chondrocytes. In the animals studied the influence of species on the intracellular lipid pattern is less striking than that of age, the site of the sample, and the position of the chondrocyte in relation to the articular surface or the perichondrium. Often more intracellular lipid is demonstrable in a cartilage in adult life than in infancy and during the early growth period. Age differences have been noted between 2-day-old and 6-month-old hamsters, between human babies and adults, and between 800 g and 2 to 3 kg rabbits. In hyaline cartilage from adults the chondrocytes nearly all contain one or more lipid drops. In the central mass of adult costal and bronchial cartilage the cells can show drops larger than the nucleus, which they can displace to the periphery of the cell. By contrast, lipid drops of this size have not been encountered in subperichondrial or in articular chondrocytes.