MALDI imaging mass spectrometry: molecular snapshots of biochemical systems
MALDI imaging mass spectrometry: molecular snapshots of biochemical systems
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DOI：
10.1038/nmeth1094
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发表时间：
2007-10-01
期刊：
影响因子：
48
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Cornett, Dale S.;Reyzer, Michelle L.;Caprioli, Richard M.
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）是一种通过直接分析薄组织切片来研究生物系统中分子分布的强大工具。MALDI-IMS在成像方法中是独一无二的，它可以在一次测量中确定数百种未知化合物的分布。我们讨论了MALDI-IMS技术的当前状态，沿着的一些最近的应用和技术发展，说明不仅其当前的能力，而且未来的潜力，该技术提供了一个更好地了解生物过程的潜在分子机制。
Matrix-assisted laser desorption/ionization ( MALDI) imaging mass spectrometry ( IMS) is emerging as a powerful tool for investigating the distribution of molecules within biological systems through the direct analysis of thin tissue sections. Unique among imaging methods, MALDI-IMS can determine the distribution of hundreds of unknown compounds in a single measurement. We discuss the current state of the art of MALDI-IMS along with some recent applications and technological developments that illustrate not only its current capabilities but also the future potential of the technique to provide a better understanding of the underlying molecular mechanisms of biological processes.