LYMPHOCYTE-T SUB-POPULATIONS IN RELATION TO IMMUNOSUPPRESSION IN MEASLES AND VARICELLA
LYMPHOCYTE-T SUB-POPULATIONS IN RELATION TO IMMUNOSUPPRESSION IN MEASLES AND VARICELLA
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DOI：
10.1128/iai.39.1.29-37.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
BIBERFELD, G
中科院分区：
文献类型：
--
作者：
ARNEBORN, P;BIBERFELD, G
采用单克隆抗体Leu 2a和Leu 3a分别针对抑制/细胞毒性t细胞亚群和辅助t细胞亚群，对麻疹或水痘患者在疾病急性期（第1周）和康复后进行淋巴细胞刺激试验和t淋巴细胞亚群测定。在两种疾病的急性期均发现植物血凝素（PHA）的低增殖反应。在所有麻疹和部分水痘患者中，对纯化的结核菌素蛋白衍生物的反应较低。在这两种感染中，自发DNA合成增加。麻疹急性期出现T淋巴细胞减少，但辅助性T淋巴细胞与抑制性T淋巴细胞/细胞毒性T淋巴细胞的比值没有变化。在水痘急性期，Leu 2阳性（抑制/细胞毒性）T细胞的百分比和绝对数量增加。淋巴细胞的大小表明该亚群被激活。水痘急性期冷冻保存的血单核细胞抑制了自身恢复期细胞的PHA反应。当对麻疹患者的细胞进行测试时，没有看到这种情况。水痘对淋巴细胞刺激反应的抑制可能部分是由抑制细胞的激活引起的；麻疹刺激反应的抑制似乎是由其他机制引起的。
Patients with measles or varicella were studied during the acute phase (1st wk) of illness and after recovery by lymphocyte stimulation tests and determinatin of T-lymphocyte subpopulations, using the monoclonal antibodies Leu 2a and Leu 3a directed at the suppressor/cytotoxic and the helper T-cell subsets, respectively. Low proliferative responses to phytohemagglutinin [PHA] were found during the acute phase of both diseases. The responses to purified protein derivative of tuberculin was low in all measles and in some varicella patients. In both infections, increased spontaneous DNA synthesis was demonstrated. In the acute phase of measles there was T lymphocytopenia but no change of the ratio between helper and suppressor/cytotoxic T lymphocytes. In the acute phase of varicella the percentage and the absolute number of Leu 2-positive (suppressor/cytotoxic) T cells were increased. The size of the lymphocytes indicated activation of this subset. Cryopreserved blood mononuclear cells from the acute phase of varicella suppressed the PHA response of autologous convalescent-phase cells. This was not seen when cells from measles patients were tested. Suppression of the lymphocyte stimulation response in varicella is probably caused, in part, by activation of suppressor cells; the suppression of the stimulation response in measles seems to be caused by other mechanisms.