Rapid single nucleotide polymorphism mapping in C-elegans -: art. no. 118
Rapid single nucleotide polymorphism mapping in C-elegans -: art. no. 118
复制标题
DOI：
10.1186/1471-2164-6-118
复制
发表时间：
2005-09-12
期刊：
影响因子：
4.4
通讯作者：
Jorgensen, EM
中科院分区：
文献类型：
--
作者：
Davis, MW;Hammarlund, M;Jorgensen, EM
背景：在C.在秀丽隐杆线虫中，单核苷酸多态性（SNP）可以作为沉默遗传标记，其应用范围从经典的二因子和三因子作图到测量整个染色体上的重组。结果：在这里，我们描述了一组48个引物对，这些引物对位于秀丽隐杆线虫SNP两侧，均匀分布在秀丽隐杆线虫上。elegans基因组，并在相同的PCR条件下工作。这组中的每个SNP改变DraI位点，从而实现快速和平行评分。我们描述了一个程序，使用这些试剂快速，可靠地映射突变。我们表明，这些技术正确映射一个已知的基因，dpy-5。然后，我们使用这些技术来映射突变的一个未知的菌株，并显示其行为表型可以同时映射到三个locus.Conclusion：在一起，试剂和方法描述的准确，快速和廉价的基因定位在C。优雅的。
Background: In C. elegans, single nucleotide polymorphisms ( SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes.Results: Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci.Conclusion: Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans.