Induction of apoptosis in human breast cancer cells by a pulsed atmospheric pressure plasma jet
Induction of apoptosis in human breast cancer cells by a pulsed atmospheric pressure plasma jet
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DOI：
10.1063/1.3462293
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发表时间：
2010-07-12
影响因子：
4
通讯作者：
Leem, S. H.
中科院分区：
文献类型：
--
作者：
Kim, Sun Ja;Chung, T. H.;Leem, S. H.
利用重复率为几万赫兹的脉冲直流电压驱动的大气压等离子体射流，我们能够诱导培养的人乳腺癌细胞(MCF-7)发生凋亡。用流式细胞仪和荧光染色方法检测等离子体处理后细胞的凋亡变化。观察到这些细胞中有很大一部分呈现出凋亡性碎裂。添加O(2)气体的氦气等离子体具有诱导细胞凋亡的作用。这种等离子体射流为人乳腺癌细胞的治疗提供了一种有效的模式。(C)2010年美国物理研究所。[DOI：10.1063/1.3462293]
By using an atmospheric pressure plasma jet driven by pulsed dc voltage with repetition rate of several tens of kilohertz, we were able to induce apoptosis in cultured human breast cancer cells (MCF-7). The apoptotic changes in cells with plasma treatment were detected by flow cytometry and fluorescence staining assay. A significant portion of these cells was observed to exhibit the apoptotic fragmentation. Helium plasma with additive O(2) gas was found to be effective in the induction of apoptosis. This plasma jet provides an effective mode of human breast cancer cell therapy. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3462293]