Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion
Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion
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DOI：
10.1128/jvi.78.11.6048-6054.2004
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发表时间：
2004-06-01
影响因子：
5.4
通讯作者：
Rottier, PJM
中科院分区：
文献类型：
--
作者：
de Haan, CAM;Stadler, K;Rottier, PJM
小鼠肝炎冠状病毒株A59刺突蛋白的切割被一种肽弗林蛋白酶抑制剂以浓度依赖性方式阻断，表明弗林蛋白酶或弗林蛋白酶样酶负责这一过程。虽然细胞-细胞融合明显受到阻止刺突蛋白裂解的影响，但病毒-细胞融合没有受到影响，表明这些事件具有不同的要求。
Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.