Deciphering the Resistance Mechanism of Tomato Plants Against Whitefly-Mediated Tomato Curly Stunt Virus Infection through Ultra-High-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)-Based Metabolomics Approaches
Deciphering the Resistance Mechanism of Tomato Plants Against Whitefly-Mediated Tomato Curly Stunt Virus Infection through Ultra-High-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)-Based Metabolomics Approaches
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DOI：
10.3390/metabo9040060
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发表时间：
2019-03
期刊：
影响因子：
4.1
通讯作者：
Leandri T Rossouw;N. Madala;F. Tugizimana;P. Steenkamp;L. L. Esterhuizen-L.;I. Dubery
中科院分区：
文献类型：
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作者：
Leandri T Rossouw;N. Madala;F. Tugizimana;P. Steenkamp;L. L. Esterhuizen-L.;I. Dubery
Begomoviruses，例如番茄卷曲矮化病毒（ToCSV），由于严重的农作物损失而造成严重的经济后果。因此，开发和筛选可能的抗性标记势在必行。虽然一些番茄品种对不同的贝戈莫病毒种类表现出不同的抗性，但在大多数情况下，抗性机制尚不完全清楚。在这项研究中，使用非靶向超高效液相色谱与基于质谱 (UHPLC-MS) 的代谢组学研究了对粉虱介导的 ToCSV 感染的抗性不同的两个近等基因番茄品系 (Solanum lycopersicum) 的反应。使用多元统计模型破译了两条线的响应。不同时间间隔的主成分分析 (PCA) 评分图显示，抗性品系对代谢组变化的反应比易感品系更快。此外，跨越一系列代谢途径的化学多样性代谢物的代谢重编程与防御反应相关。生物标志物主要包括与奎尼酸、半乳糖二酸和葡萄糖缀合的羟基肉桂酸。次要成分包括苯类、黄酮类和甾体配糖生物碱。有趣的是，当减少到代谢物水平时，受感染植物的植物化学反应非常相似。然而，耐药表型与响应感染而部署的羟基肉桂酸衍生物密切相关。此外，抵抗线能够产生更强、更快的反应。
Begomoviruses, such as the Tomato curly stunt virus (ToCSV), pose serious economic consequences due to severe crop losses. Therefore, the development and screening of possible resistance markers is imperative. While some tomato cultivars exhibit differential resistance to different begomovirus species, in most cases, the mechanism of resistance is not fully understood. In this study, the response of two near-isogenic lines of tomato (Solanum lycopersicum), differing in resistance against whitefly-mediated ToCSV infection were investigated using untargeted ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS)-based metabolomics. The responses of the two lines were deciphered using multivariate statistics models. Principal component analysis (PCA) scores plots from various time intervals revealed that the resistant line responded more rapidly with changes to the metabolome than the susceptible counterpart. Moreover, the metabolic reprogramming of chemically diverse metabolites that span a range of metabolic pathways was associated with the defence response. Biomarkers primarily included hydroxycinnamic acids conjugated to quinic acid, galactaric acid, and glucose. Minor constituents included benzenoids, flavonoids, and steroidal glycoalkaloids. Interestingly, when reduced to the level of metabolites, the phytochemistry of the infected plants’ responses was very similar. However, the resistant phenotype was strongly associated with the hydroxycinnamic acid derivatives deployed in response to infection. In addition, the resistant line was able to mount a stronger and quicker response.