Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni.
Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni.
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幽门弯曲杆菌外膜蛋白的鉴定以及幽门弯曲杆菌和空肠弯曲杆菌之间的抗原交叉反应性。
DOI：
10.1099/00221287-133-1-163
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Newell
中科院分区：
文献类型：
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作者：
D. Newell
用SDS-PAGE电泳和~（125）I表面标记全菌鉴定了4株胃弯曲菌样生物幽门弯曲菌的外膜和表面暴露蛋白。虽然恒定的外膜蛋白（分子量为61，54和31 kDa），在这些菌株中观察到，几个变量125 I标记的表面蛋白被检测到。C.幽门螺杆菌似乎不像C. jejuni和C.杆菌推定的鞭毛蛋白从分离的鞭毛和酸可提取的表面材料，并通过免疫印迹与抗鞭毛抗体。用抗-C免疫印迹法观察到几种主要的蛋白抗原。幽门螺杆菌抗血清。这些抗原中至少有两种与抗C交叉反应。空肠抗血清这种交叉反应似乎主要由鞭毛抗原引起。然而，一种主要蛋白抗原（61 kDa）与C.因此，在C. jejuni的特异性诊断的血清学测试中可能有用。幽门感染
The outer membrane and surface exposed proteins of four strains of the gastric Campylobacter-like organism Campylobacter pyloridis were identified by SDS-PAGE of Sarkosyl-insoluble membranous material and 125I-surface-labelled whole bacteria. Although constant outer membrane proteins (molecular mass 61, 54 and 31 kDa) were observed in these strains, several variable 125I-labelled surface proteins were detected. C. pyloridis does not appear to express a single surface-exposed major outer membrane protein like that of C. jejuni and C. coli. Putative flagella proteins were identified from isolated flagella and acid-extractable surface material and by immunoblotting with anti-flagella antibodies. Several major protein antigens were observed by immunoblotting with anti-C. pyloridis antisera. At least two of these antigens cross-reacted with anti-C. jejuni antiserum. This cross-reaction appears to be caused primarily by flagellar antigens. However, one major protein antigen (61 kDa) was not cross-reactive with C. jejuni and may, therefore, be useful in serological tests for the specific diagnosis of C. pyloridis infections.