Genomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales.
Genomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales.
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DOI：
10.1186/1471-2180-10-37
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发表时间：
2010-02-08
期刊：
影响因子：
4.2
通讯作者：
Vasconcelos AT
中科院分区：
文献类型：
--
作者：
Carvalho FM;Souza RC;Barcellos FG;Hungria M;Vasconcelos AT
属于根瘤菌目的物种是有趣的和广泛的研究，包括与豆科植物共生时具有固氮能力的细菌和对动物和植物的病原细菌。致病性和共生性根瘤菌目所采取的策略之间的相似性已经被描述，以及与水平基因转移事件相关的高度变异性。虽然众所周知，染色体重排、突变和基因水平转移会影响细菌基因组的动态，但在根瘤菌目中，决定致病或共生生活方式的情景尚不清楚，并且很少有这些原核微生物之间的比较基因组研究试图描绘共生和致病的进化特征。非共生固氮菌和参与生物修复的细菌更接近共生菌和病原菌，可能有助于根瘤菌的起源和祖先基因以及发生的基因流。通过对19种根瘤菌的基因组比较，包括固氮菌、生物修复菌和病原菌的基因组比较，得到33个与固氮菌和病原菌共同的簇，15个与所有固氮菌和生物修复菌共同的簇，13个与部分固氮菌和生物修复菌共同的簇，01个与部分共生菌共同的簇，而01簇仅在所分析的部分病原体中发现。对所有菌株进行生物固氮试验，共获得77个共有基因，其中17个与生物固氮和致病相关。修复，Nif，Nod，Vir和Trb的系统发育重建显示可能的水平基因转移事件，分组不同表型的物种。在病原体和共生体中存在的共生和毒力基因似乎并不是这些微生物生活方式进化的唯一决定因素，尽管它们可能在宿主感染的共同阶段起作用。对参与这些过程的许多不同操纵子的系统发育分析强调了水平基因转移事件在共生和致病相似性中的相关性。
Species belonging to the Rhizobiales are intriguing and extensively researched for including both bacteria with the ability to fix nitrogen when in symbiosis with leguminous plants and pathogenic bacteria to animals and plants. Similarities between the strategies adopted by pathogenic and symbiotic Rhizobiales have been described, as well as high variability related to events of horizontal gene transfer. Although it is well known that chromosomal rearrangements, mutations and horizontal gene transfer influence the dynamics of bacterial genomes, in Rhizobiales, the scenario that determine pathogenic or symbiotic lifestyle are not clear and there are very few studies of comparative genomic between these classes of prokaryotic microorganisms trying to delineate the evolutionary characterization of symbiosis and pathogenesis. Non-symbiotic nitrogen-fixing bacteria and bacteria involved in bioremediation closer to symbionts and pathogens in study may assist in the origin and ancestry genes and the gene flow occurring in Rhizobiales. The genomic comparisons of 19 species of Rhizobiales, including nitrogen-fixing, bioremediators and pathogens resulted in 33 common clusters to biological nitrogen fixation and pathogenesis, 15 clusters exclusive to all nitrogen-fixing bacteria and bacteria involved in bioremediation, 13 clusters found in only some nitrogen-fixing and bioremediation bacteria, 01 cluster exclusive to some symbionts, and 01 cluster found only in some pathogens analyzed. In BBH performed to all strains studied, 77 common genes were obtained, 17 of which were related to biological nitrogen fixation and pathogenesis. Phylogenetic reconstructions for Fix, Nif, Nod, Vir, and Trb showed possible horizontal gene transfer events, grouping species of different phenotypes. The presence of symbiotic and virulence genes in both pathogens and symbionts does not seem to be the only determinant factor for lifestyle evolution in these microorganisms, although they may act in common stages of host infection. The phylogenetic analysis for many distinct operons involved in these processes emphasizes the relevance of horizontal gene transfer events in the symbiotic and pathogenic similarity.
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DOI：
10.1073/pnas.0400975101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Boussau, B;Karlberg, EO;Andersson, SGE
通讯作者：
Andersson, SGE
影响因子：
4.4
作者：
Barcellos, Fernando Gomes;Menna, Pamela;Hungria, Mariangela
通讯作者：
Hungria, Mariangela
影响因子：
3.2
作者：
Jones, Kathryn M.;Lloret, Javier;Walker, Graham C.
通讯作者：
Walker, Graham C.
影响因子：
3.4
作者：
Guerrero, G;Peralta, H;Aguilar, A;Díaz, R;Villalobos, MA;Medrano-Soto, A;Mora, J
通讯作者：
Mora, J
影响因子：
3.2
作者：
den Hartigh, Andreas B.;Rolan, Hortensia G.;Tsolis, Renee M.
通讯作者：
Tsolis, Renee M.