Characterization of Pseudomonas aeruginosa isolates from dogs and cats in Japan: current status of antimicrobial resistance and prevailing resistance mechanisms
Characterization of Pseudomonas aeruginosa isolates from dogs and cats in Japan: current status of antimicrobial resistance and prevailing resistance mechanisms
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DOI：
10.1111/j.1348-0421.2011.00416.x
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发表时间：
2012-02-01
影响因子：
2.6
通讯作者：
Takahashi, Toshio
中科院分区：
文献类型：
--
作者：
Harada, Kazuki;Arima, Sayuri;Takahashi, Toshio
为了解铜绿假单胞菌对抗生素的敏感性及耐药机制，从日本猫犬分离到73株铜绿假单胞菌。对奥比沙星、恩诺沙星、环丙沙星、头孢替辛、氨曲南、庆大霉素耐药率分别为34.2%、31.5%、20.5%、17.8%、12.3%、4.1%。外排泵抑制剂对头孢噻肟、奥比沙星和恩诺沙星的耐药程度有很大影响，提示外排泵的过度表达是导致这些耐药性的原因之一。值得注意的是，即使在靶点没有突变的分离株中也观察到奥比沙星和恩诺沙星耐药。这是首次报道来自日本同伴动物的铜绿假单胞菌对头孢菌素和氟喹诺酮类药物的耐药性。
Seventy-three Pseudomonas aeruginosa isolates were collected from dogs and cats in Japan to investigate antimicrobial susceptibility and resistance mechanisms to anti-pseudomonal agents. Resistance rates against orbifloxacin, enrofloxacin, ciprofloxacin, cefotaxime, aztreonam and gentamicin were 34.2, 31.5, 20.5, 17.8, 12.3 and 4.1%, respectively. The degree of resistance to cefotaxime, orbifloxacin, and enrofloxacin was greatly affected by efflux pump inhibitors, indicating overexpression of efflux pump contributes to these resistances. Notably, orbifloxacin and enrofloxacin resistance was observed even in isolates without mutations in the target sites. This is the first report on cephalosporin- and fluoroquinolone-resistant isolates of P. aeruginosa from Japanese companion animals.