Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin
复制标题
髓系白血病细胞分化过程中收缩蛋白的变化。
作者：
K. Nagata;J. Sagara;Y. Ichikawa
在骨髓性白血病细胞系M1的分化过程中，细胞肌动蛋白发生了定量和定性的变化，M1是用条件培养基（CM）诱导的。CM作用3d后，M1细胞向巨噬细胞分化并丧失有丝分裂活性，肌动蛋白含量、F-肌动蛋白占总肌动蛋白的比例及肌动蛋白合成均增加。分化前后G-actin的抗凋亡能力差异较大。与来自未处理的Ml细胞的肌动蛋白相比，从CM处理的细胞收获的肌动蛋白显示出更大的粘附能力，这取决于MgCl 2和/或KCl和蛋白质的浓度增加。从Mml细胞系（巨噬细胞系）收获的肌动蛋白在有或没有CM处理的情况下具有特别高的可聚合性。相反，来自D-亚系的肌动蛋白对CM不敏感，几乎没有聚合。
Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.