The N-hydroxysuccinimide ester of Boc-[S-(3-nitro-2-pyridinesulfenyl)]-cysteine: a heterobifunctional cross-linking agent.
The N-hydroxysuccinimide ester of Boc-[S-(3-nitro-2-pyridinesulfenyl)]-cysteine: a heterobifunctional cross-linking agent.
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Boc-[S-(3-硝基-2-吡啶硫基)]-半胱氨酸的 N-羟基琥珀酰亚胺酯：一种异双功能交联剂。
DOI：
10.1016/0006-291x(85)91912-6
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发表时间：
1985
影响因子：
3.1
通讯作者：
Matsueda,GR
中科院分区：
文献类型：
--
作者：
Bernatowicz,MS;Matsueda,GR
用半胱氨酸的S-（3-硝基-2-吡啶磺酰基）衍生物通过二硫键将合成的含半胱氨酸肽单向偶联到白蛋白上。该方法采用Boc-[S-（3-硝基-2-吡啶磺酰基）]-半胱氨酸的n -羟基琥珀酰亚胺酯作为异双功能交联剂，这是一种用于肽合成的受保护氨基酸衍生物。共轭物中的二硫键是由游离硫醇与S-（3-硝基-2-吡啶磺酰基）基团反应形成的。牛白蛋白以这种方式与几种从人纤维蛋白衍生的合成肽偶联。对这些偶联物的氨基酸分析表明，每个蛋白质分子结合了6到11个肽分子。
Synthetic cysteine-containing peptides were unidirectionally conjugated to albumin via disulfide bonds using the S-(3-nitro-2-pyridinesulfenyl) derivative of cysteine. This method employs the N-hydroxysuccinimide ester of Boc-[S-(3-nitro-2-pyridinesulfenyl)]-cysteine, a protected amino acid derivative used in peptide synthesis, as a heterobifunctional cross-linking agent. The disulfide bonds in the conjugates are formed by the reaction of free thiols with S-(3-nitro-2-pyridinesulfenyl) groups. Bovine albumin was conjugated in this manner to several synthetic peptides derived from human fibrin. Amino acid analysis of these conjugates demonstrated incorporations of from 6 to 11 peptide molecules per molecule of protein.