Total Phospholipids in Edible Oils by In-Vial Solvent Extraction Coupled with FTIR Analysis
Total Phospholipids in Edible Oils by In-Vial Solvent Extraction Coupled with FTIR Analysis
复制标题
通过瓶内溶剂萃取结合 FTIR 分析测定食用油中的总磷脂
DOI：
10.1021/jf404880v
复制
发表时间：
2014-04-09
影响因子：
6.1
通讯作者：
van de Voort, Fred R.
中科院分区：
文献类型：
--
作者：
Meng, Xianghe;Ye, Qin;van de Voort, Fred R.
建立了一种简单的测定食用油中总磷脂（TPL）含量的方法，该方法采用甲醇/乙腈（MeOH/ACN）原位萃取，傅立叶变换红外（FTIR）光谱分析。在25 μ m CaF 2池中提取物的光谱分析表明，仅使用二阶导数光谱中1243 cm（-1）处的不对称磷酸二酯PO 2-伸缩带进行测量，就足以准确测量TPL，同时遇到最小的共提取甘油三酯干扰。仅使用磷脂酰胆碱（PC）作为代表性磷脂标准品设计FTIR校准光谱，覆盖0-50000 μ g/g TPL的范围，并掺入1：1 MeOH/ACN中，能够跟踪添加的PC，SD为
A simple procedure for the determination of total phospholipids (TPL) in edible oils was developed by combining a single-step, in situ methanol/acetonitrile (MeOH/ACN) extraction of the oil sample followed by Fourier transform infrared (FTIR) spectroscopic analysis of the extract. Spectral analysis of extracts in a 25 mu m CaF2 cell obtained using 1:1 MeOH/ACN added to oil in a 2:1 ratio indicated that measurements made using only the asymmetric phosphate diester PO2- stretching band at 1243 cm(-1) in second-derivative spectra were sufficient for the accurate measurement of TPL with minimal coextracted triglyceride interferences being encountered. FTIR calibration spectra were devised using only phosphatidylcholine (PC) as a representative phospholipid standard, covering a range of 0-50000 mu g/g TPL and spiked into 1:1 MeOH/ACN, capable of tracking the added PC with an SD of